Capillary mass spectrometry

CLP = contract laboratory program EC = electron capture detection FSCC-MS/DS = fused silica capillary, mass spectrometry/data system GC = gas chromatography HPLC-UV = high-resolution liquid chromatography - ultraviolet detection 2-NP = 2-nitrophenol 4-NP = 4-nitrophenol... 

Matsumoto K, Nagata S, Hattori H, Tsuge S. Development of directly coupled supercritical fluid chromatography with packed capillary-mass spectrometry with atmospheric pressure chemical ionization. J Chromatogr 1992 605 87-94. Morgan DG, Norwood DL, Fisher DL, Moseley MA III. Directly coupled packed column SFC with APCI/MS. Poster and abstract presented at American Society for Mass Spectrometry. Portland, OR, 1996... 

Interest in this method has decreased since advances made in gas chromatography using high-resolution capillary columns (see article 3.3.3.) now enable complete identification by individual chemical component with equipment less expensive than mass spectrometry. 

Detectors Most of the detectors used in HPLC also find use in capillary electrophoresis. Among the more common detectors are those based on the absorption of UV/Vis radiation, fluorescence, conductivity, amperometry, and mass spectrometry. Whenever possible, detection is done on-column before the solutes elute from the capillary tube and additional band broadening occurs. 

CE/MS. capillary electrophoresis and mass spectrometry used as a combined technique... 

Jaeger, H., Capillary Gas Chromatography Mass Spectrometry in Medicine and Pharmacology, Huetig, New York, 1987. 

Identification of stmctures of toxic chemicals in environmental samples requires to use modern analytical methods, such as gas chromatography (GC) with element selective detectors (NPD, FPD, AED), capillary electrophoresis (CE) for screening purposes, gas chromatography/mass-spectrometry (GC/MS), gas chromatography / Fourier transform infra red spectrometry (GC/FTIR), nucleai magnetic resonance (NMR), etc. 

A. J. Tomlinson and S. Naylor, Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry foi analysis of peptide mixtures, J. Capillary Electrophoresis 225-233 (1995). 

ONLINE REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-CAPILLARY ZONE ELECTROPHORESIS - MASS SPECTROMETRY... 

H. J. Goites, B. M. Bell, G. D. Pfeiffer and J. D. Graham, Multidimensional chromatography using on-line coupled microcolumn size exclusion cliromatography-capillary gas chromatography-mass spectrometry for determination of polymer additives , J. Microcolumn Sep. 1 278-288. (1989)... 

The Tools of Proteomics A variety of methods and techniques including two-dimensional gel electrophoresis (2DE), capillary liquid chromatography, stable isotope labeling, and mass spectrometry has been developed for qualitative and quantitative protein... 

The combination of chromatography and mass spectrometry (MS) is a subject that has attracted much interest over the last forty years or so. The combination of gas chromatography (GC) with mass spectrometry (GC-MS) was first reported in 1958 and made available commercially in 1967. Since then, it has become increasingly utilized and is probably the most widely used hyphenated or tandem technique, as such combinations are often known. The acceptance of GC-MS as a routine technique has in no small part been due to the fact that interfaces have been available for both packed and capillary columns which allow the vast majority of compounds amenable to separation by gas chromatography to be transferred efficiently to the mass spectrometer. Compounds amenable to analysis by GC need to be both volatile, at the temperatures used to achieve separation, and thermally stable, i.e. the same requirements needed to produce mass spectra from an analyte using either electron (El) or chemical ionization (Cl) (see Chapter 3). In simple terms, therefore, virtually all compounds that pass through a GC column can be ionized and the full analytical capabilities of the mass spectrometer utilized. 

Liquid Chromatography-Mass Spectrometry Vaporizer Seated capillary Skimmer... 

Figure 5.54 Structures of Praziquantel and its metabolites, cis- and fraw5-4-hydroxy-praziquantel. Reprinted from 7. Chromatogr., B, 708, Lerch, C. and Blaschke, G., Investigation of the stereoselective metabolism of Praziquantel after incubation with rat liver microsomes by capillary electrophoresis and liquid chromatography-mass spectrometry , 267-275, Copyright (1998), with permission from Elsevier Science.

Eichelberger JW, Kerns EH, Olynyk P, et al. 1983. Precision and accuracy in the determination of organics in water by fused silica capillary column gas chromatography/mass spectrometry and packed column gas chromatography/mass spectrometry. Anal Chem 55 1471-1479. 

DALLUGE J J, NELSON B c, THOMAS J B, WELCH M J and SANDER L c (1997) Capillary liquid chromatography/electrospray mass spectrometry for the separation and detection of catechins in green tea and human plasma , Rapid Commun Mass Spectrom, 11, 1753-6. 

Hutton, T. and Major, H.J., Characterizing biomolecnles by electrospray ionization-mass spectrometry coupled to liqnid chromatography and capillary electrophoresis, Biochem. Soc. Trans., 23, 924, 1995. 

The development of new fiber coatings in the near future should further improve the specificity of SPME and overcome some of the observed matrix effects. Quantification by stable isotope dilution gas chromatography/mass spectrometry (GC/MS) may assist in improving analytical performance. Along with the possible application of micro LC and capillary LC columns to in-tube SPME, the development of novel derivatization methods and the potential for the analysis of fumigant pesticides, SPME appears to be a technique with a future in the analysis of pesticide residues in food. 

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