Capillary electrophoresis carbohydrates

Kwon C, Jung S. Stereoisomeric separation of some flava-nones using highly succinate-substituted a-cyclosophoro-octadecaoses as chiral additives in capillary electrophoresis. Carbohydr. Res. 2011 346 133-139. 

Tran, A. D., Park, S., Lisi, P. J., Huynh, O. T., Ryall, R. R., and Lane, P. A., Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis. Effects of pH, buffer type and organic additives,. Ckromatogr., 542, 459, 1991. 

Liu, J., Shirota, O., Wiesler, D., and Novotny, M., Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis, Proc. Nat. Acad. Sci., 88, 2302, 1991. 

Figure 3.12 Metabolic profiling by capillary electrophoresis, (a) Comparative carbohydrate profiles of M. truncatula tissue obtained using 4-aminobenzonitrile derivatization, capillary electrophoresis with a 150 mM borate buffer, pH = 9, and on-column UV detection at 214 nm. (b) Anion profile from M. truncatula using capillary electrophoresis and indirect UV detection. The separation buffer was 5 mM K2C1O4, 1% Waters OFM-Anion BT, pH 8.0.

PAULUS, A., KLOWCKOW-BECK, A., Analysis of carbohydrates by capillary electrophoresis. In Chromatographia CE Series, (K. D. Altria, ed.), Vol. 3, Vieweg Sohn, Wiesbanden, Germany, 1999, pp. 93-170. 

EL RASSI, Z., Recent developments in capillary electrophoresis and capillary electrochromatography of carbohydrate species, Electrophoresis, 1999, 20, 3134-3144. 

Paulus, A., and Klockow-Beck, A. (1999). Analysis of Carbohydrates by Capillary Electrophoresis, Vieweg, Wiesbaden. 

Taverna, M., Baillet, A., Biou, D., Schluter, M., Werner, R., and Terrier, D. (1992). Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high-performance capillary electrophoresis. Electrophoresis 13, 359—366. 

Lurie, L, Hays, P., and Valentino, A. (2006). Analysis of carbohydrates in seized heroin using capillary electrophoresis. J. Forensic Sci. 51, 39—44. 

El Rassi, Z. (1996). High Performance Capillary Electrophoresis of Carbohydrates, Beckman Instruments., p. 102... 

Campa, C., Coslovi, A., Flamigni, A., and Rossi, M. (2006). Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates a tabulated review. Electrophoresis 27, 2027-2050. 

Townsend, R. R. (1995). Carbohydrate Analysis High-Performance Liquid Chromatography and Capillary Electrophoresis, Elsevier, New York. 

Capillary electrophoresis has found use in the biotechnology industry for structural analysis of recombinant proteins. The high resolving power of CE for charged analytes makes it a powerful tool for the analysis of tryptic digests. Therefore, many of the techniques given here, such as the determination of thiols, carbohydrates, and amino acids, will be employed for this purpose. 

The reader is directed to Ref. 5, which makes an interesting comparison between HPLC and other analytical methodologies for the determination of carbohydrates in foods. Additionally, notable progress has been made in the application of high-performance capillary electrophoresis (HPCE) in this field (8-11). However, given the scope of this chapter, we will focus on the advantages and drawbacks of other chromatographic techniques versus HPLC. 

H.-L. Lee and S.-C. Chen, Microchip capillary electrophoresis with am-perometric detection for several carbohydrates, Talanta, 64 (2004) 210-216. 

N. E. Hebert, W.G. Ruhr and S.A. Brazill, Microchip capillary electrophoresis coupled to sinusoidal voltammetry for the detection of native carbohydrates, Electrophoresis, 23 (2002) 3750-3759. 

J. Wang, G. Chen, M. Wang and M.P. Chatrathi, Carbon-nanotube/cop-per composite electrodes for capillary electrophoresis microchip detection of carbohydrates, Analyst, 29 (2004) 512-515. 

A. D. Zamfir, N. Dinca, E. Sisu, and J. Peter-Katalinic, Copper-coated microsprayer interface for on-line sheathless capillary electrophoresis electrospray mass spectrometry of carbohydrates,. / Sep. Sci., 29 (2006) 414 422. 

Baldwin, R.P., Electrochemical determination of carbohydrates Enzyme electrodes and amperometric detection in liquid chromatography and capillary electrophoresis. J. Pharm. Biomed. Anal. 1999, 19, 69-81. 

The quantification of specific sugars and total carbohydrates should be performed in each lot. In some cases, IEF and capillary electrophoresis can be alternative methods for determining the percentage of each isoform in a product sample lot. It is often important to determine the isoform profile for each lot prior to release. It would be ideal, but not always possible, to relate the isoform profile to the specific activity of the product. 

---