Carbohydrates electrophoresis

SCHWAIGER, H., OEFNER, P.J., HUBER, C GRILL, E., BONN, G.K., Capillary zone electrophoresis and micellar electrokinetic chromatography of 4-aminobenzonitrile carbohydrate, Electrophoresis, 1994,15, 941-952. 

N. E. Hebert, W.G. Ruhr and S.A. Brazill, Microchip capillary electrophoresis coupled to sinusoidal voltammetry for the detection of native carbohydrates, Electrophoresis, 23 (2002) 3750-3759. 

When HA samples contain only several disaccharide repeating units (from 10- to 12-mers), PAGE is not appropriate. An alternative gel electrophoretic procedure known as Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) is more suited for these cases. In FACE technique, the sample is derivatized with a fluorescent group at the reducing end-group prior to electrophoresis. The FACE electrophoretic method was also described in detail by Cowman and Mendichi [270]. 

Suzuki, S. and Honda, S. A tabulated review of capillary electrophoresis of carbohydrates. Electrophoresis, 19, 2539, 1998. 

El Rassi, Z. and Mechref, Y. Recent advances in capillary electrophoresis of carbohydrates. Electrophoresis, 17, 275, 1996. 

Calabro, A. et al. Fluorophore-assisted carbohydrate electrophoresis, FACE of glycosaminoglycans. Osteoarthritis Cartilage, 9 (SupplA), SI6, 2001. 

MoreU, M. K., Samnel, M. S. and O Shea, M. G Analysis of starch structure using fluorophore-assisted carbohydrate electrophoresis. 19, 2603, 1998. 

Guo-Fu H (1995) Fluorophore-assisted carbohydrate electrophoresis technology and applications. Journal of Chromatography A 705 89-103. 

S. S. Basu, D. Dastgheibhosseini, G. Hoover, Z. X. Li, and S. Basu, Analysis of glycosphingolipids by fluorophore-assisted carbohydrate electrophoresis using ceramideglycanase from Afercenar/amercenana, Anal. Biochem. 222 270-274 (1994). 

A review has appeared on Tluorophore-assisted carbohydrate electrophoresis (FACE) by which fluorescently-labelled carbohydrates are separated on polyacrylamide gels and a charged-coupled device camera is used to detect and quantify the products. ... 

Robbe, C. Capon, C. Flahaut, C. Michalski, J.-C. Microscale analysis of mucin-type O-glycans by a coordinated fluorophore-assisted carbohydrate electrophoresis and mass spectrometry approach. Electrophoresis 2003, 24, 611-621. 

---