Carbohydrate-mediated

Tran, A. D., Park, S., Lisi, P. J., Huynh, O. T., Ryall, R. R., and Lane, P. A., Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis. Effects of pH, buffer type and organic additives,. Ckromatogr., 542, 459, 1991. 

P. Sears, C.-H. Wong, Carbohydrate Mimetics A New Strategy for Tackling the Problem of Carbohydrate-Mediated Biological Recognition , Angew. Chan. Int. Ed 1999, 38, 2300-2324. 

Special emphasis is placed on the carbohydrate-mediated cell - target system interaction by describing hints and pitfalls of assays for cytoadhesion, specificity, cytoinvasion, and cytoevasion. In addition, basic considerations are presented to discriminate between active and passive uptake as well as to detect lysosomal accumulation. Finally, the pros and cons of two useful analytical techniques, namely, flow cytometry and confocal laser scanning microscopy, are described in detail. 

Taverna, M., Baillet, A., Biou, D., Schluter, M., Werner, R., and Terrier, D. (1992). Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high-performance capillary electrophoresis. Electrophoresis 13, 359—366. 

Carbohydrate-mediated cell adhesion is an important event which can be initiated by tissue injury or infection and is involved in metastasis. One such adhesion process is the interaction between the glycoprotein E-selectin and oligosaccharides on the surface of neutrophils (white blood cells). The ligand that E-selectin recognizes is the tetrasaccharide sialyl Lewis X (SLe ). Since SLe competes with white blood cells for binding to E-selectin, thus inhibiting the adhesion process, it may useful as an anti-inflammatoiy and anticancer agent. 

Jones, M. N. Carbohydrate-mediated liposomal targeting and drug delivery. Adv. Drug Del. Rev. 13 215-250, 1994. 

K. Schnatbaum and H. J. Schafer, Electroorganic synthesis 66 Selective anodic oxidation of carbohydrates mediated by TEMPO, Synthesis (1999) 864-872. 

Paulson JC (1992) Selectin/carbohydrate mediated adhesion of leukocytes. In Harlan JM, Liu DY (eds) Adhesion Its role in inflammatory disease. Freeman, New York, p 19... 

Fukuda M (1994) Carbohydrate-mediate adhesion of tumor cells. In Bock K (ed) Complex carbohydrates in drug research, Alfred Benzon Symposium. Mungsgaard, Copenhagen, p 353... 

Focarelli, R., La Sala, G. B., Balasini, M., and Rosati, F. (2001) Carbohydrate-mediated sperm-egg interaction and species specificity a clue from the Unio elon-gatulus model. Cells Tissues Organs 168(1-2), 76-81. 

Feizi T, Carbohydrate-mediated recognition systems in innate immunity, Immunol. Rev., 173 79-88, 2000. 

R. Takano, T. Kanda, K. Hayashi, K. Yoshida, and S. Hara, Desulfation of sulfated carbohydrates mediated by silylating reagents, J. Carbohydr. Chem., 14 (1995) 885-888. 

In a subsequent investigation by the author [ 1 ] multivalent glycopolymers with chain-terminating binding groups, (I), were prepared and used in carbohydrate-mediated biomolecular recognition processes. 

Wong C-H (1995) Topochemistry and inhibition of carbohydrate-mediated cell adhesion. In JoUes G,Dean PM (eds) New perspectives in drug design. Academic Press, London, p 35... 

Kannagi, R. (1997). Carbohydrate-mediated cell adhesion involved in hematogenous metastasis of cancer. Glycoconj. J. 14, 577-584. 

Sears, P, Wong, C-H, Carbohydrate mimetics a new strategy for tackling the problem of carbohydrate-mediated biological recognition, Angew. Chem. Int. Ed. Engl., 38, 2317, 1999. 

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