Cancer cell, gene expression

Frederiksen KS, Abrahamsen N, Cristiano RJ, Damstrup L, Poulsen HS (2000) Gene delivery by an epidermal growth factor/DNA polyplex to small cell lung cancer cell lines expressing low levels of epidermal growth factor receptor. Cancer Gene Ther 7 262-268... 

In other experiments, the effects of 16 flavonoids and 5 isoflavones on the drug accumulation were compared in human MDR1 gene-transfected mouse lymphoma cells and MRP-expressing human breast cancer cells. The expression of P-gp 170 and P-gp 190 was confirmed by an immunocytochemical method [14,16]. 

Radinsky, R. (1995a) Modulation of tumor cell gene expression and phenotype by the organ specific metastatic environment. Cancer Metastasis Rev. 14, 323-338. 

In prostate cancer cells miR-154 expression is suppressed, and CCND2 (cyclin D2) is overexpressed. The EMT activity of HMGA2 is inhibited by forced expression of miR-154. Epithelial cancer cells dominantly express cadherin mes-enchymally transformed adenocarcinoma cells (prostate cancer cells) overexpress vimentin [2175-2177]. So far no reports could be found showing that reactivated retrotransposon-LTRs activate the SALL4 and/or Hmga2 genes but may be these factors were not directly looked for. 

Notamicola M et al (2011) Effects of olive oil polyphenols on fatty acid synthase gene expression and aeTivity in human colorectal cancer cells. Genes Nutr 6 63-69... 

The CaR regulates numerous biological processes, including the expression of various genes (e.g., PTH) the secretion of hormones (PTH and calcitonin), cytokines (MCP-1), and calcium (e.g., into breast milk) the activities of channels (potassium channels) and transporters (aquaporin-2) cellular shape, motility (of macrophages), and migration cellular adhesion (of hematopoietic stem cells) and cellular proliferation (of colonocytes), differentiation (of keratinocytes), and apoptosis (of H-500 ley dig cancer cells) [3]. 

Beere, H.M., Morimoto, R.I., Hickman, J.A. (1993). Investigations of mechanisms of drug-induced changes in gene expression N methylformamide-induced changes in synthesis of the M(r) 72,000 constitutive heat shock protein during commitment of HL-60 cells to granulocyte differentiation. Cancer Res. 53, 3034—3039. 

The test system was considerably less sensitive to endosulfan when mouse ER, rather than human ER, was used to mediate (3-gal activity (Ramamoorthy et al. 1997). In similar assays, endosulfan at 10 jM had no effect on (3-gal activity in yeast Saccharomyces) transfected with either the human or rainbow trout ER (Andersen et al. 1999). In addition, no effect was observed on transcriptional activation of HeLa cells transfected with plasmids containing an estrogen receptor as a responsive element (Shelby et al. 1996). Endosulfan also did not induce transient reporter gene expression in MCF-7 human breast cancer cells at an incubation concentration of 2.5 pM (Andersen et al. 1999). Maximum endosulfan-induced ER-mediated luciferase reporter gene expression occurred in vitro in a T47D human breast adenocarcinoma cell line at approximately 10 pM, while 50% expression of luciferase occurred at about 5.9 pM the maximum expression was approximately 59% of the effect from exposure to 0.03 nM estradiol (0.00003 pM) (Legler et al. 1999). Luciferase expression from combined treatment with endosulfan and dieldrin was additive over concentrations ranging from 3 to 8 pM. 

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