Burkitt’s lymphoma cell lines

Gurtsevitch, Y.E., G.T. O Conor, and G.M. Lenoir. Burkitt s lymphoma cell lines reveal different degrees of tumorigenicity in nude mice. Int J Cancer 41 87-95, 1988. 

Vimentins are class-III intermediate filaments (IF) found in various nonepithehal cells, in particular in mesenchymal cells. These genes are highly expressed in fibroblasts, but also, though in a lesser extent, in T and B lymphocytes, while little or no expression occurs in Burkitt s lymphoma cell lines. 

In support of the EP pol model, genetic studies ivith knockout mice have revealed that two additional EP polymerases, pol t and pol might participate in SHM. Deficiencies of pol t in a Burkitt s lymphoma cell line dramatically impair the mutation frequency increase on activation of these cells, despite the presence of AID, and it was restored by overexpression of the polymerase (Faili et aL, 2002). However, a conflicting report from mice deficient for pol t indicates no change in the frequency of SHM (McDonald et al., 2003). 

D Souza B, Rowe M, Walls D (2000) The bfl-1 gene is transcriptionally upregulated by the Epstein-Barr virus LMPl, and its expression promotes the survival of a Burkitt s lymphoma cell line. J Virol 74 6652-6658... 

Burkitt s lymphoma cell line, and CCRF-CEM cells of human T cell lymphoblast-like cell line. 

Foremost among these assays is the Raji cell assay developed by The-ofilopoulos and co-workers (T15). The Raji cell line, derived from a patient with Burkitt s lymphoma, lacks surface immunoglobulin, has Fc receptors that bind monomeric IgG only weakly, and possesses numerous receptors for Clq, C3b/C4b, C3d, and other complement components (G23, S4, S27, S28, T8, T12). Immune complexes which have fixed complement bind to... 

Human B-lymphoblastoid cell line Namalwa is derived from a Burkitt s lymphoma tumour biopsy and can be grown for an indefinitely long period of time (Klein et al., 1979 Biliau et ah, 1973). Although they contain Epstein-Barr (EB) nuclear antigen, Namalwa cells do not release EB virus. The cell line is used for the production of IFN-a. Namalwa cells can be induced to produce IFN by adding Sendai virus to cells pretreated with 2 mM sodium butyrate (Baker et al, 1980 Johnston, 1980) and can be primed with Sendai virus in order to increase the levels of IFN-a produced. 

Raji cells, a latently infected cell line derived from Burkitt s lymphoma, contain constant EBV genome copy numbers (60 copies/cell). One can construct a standard linear curve by using various amounts of Raji cell DNA attached to nitrocellulose filters and hybridizing to the EBV cRNA probe as described above. EBV genome copy numbers can then be calculated, based on the net hybridized counts from the same amount of itaji DNA and drug-treated P3HR-1(LS) DNA. 

The Epstein-Barr virus (EBV) is a B-cell lymphotropic herpesvirus that induces a usually asymptomatic infection and is carried by more than 90% of adults. However, EBV is the causative agent for Burkitt s lymphoma and nasopharyngeal carcinoma and is involved in a number of acquired immunodeficiency syndrome (AIDS)-associated lymphomas. EBV can induce immortalization of B cells in vitro to generate lymphoblastoid cell lines (LCLs), a model for the carcinogenic potential... 

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