Lymphoma lines

The mechanism by which Cell-Tak protein accomplishes this efficiency at the molecular level is addressed with the observation that different cell types attach with the same kinetics (Figure 2A). The lymphoma line (U-937), BHK cells, and bovine corneal endothelial cells attach with varying efficiencies to plastic (Figure 2B) but exhibit the same rate and overall efficiency on Cell-Tak protein. The attachment lacks the specificity that would indicate receptor-mediated attachment and supports the roll of nonspecific interactions. Moreover, other data (not shown) suggest that the attachment of cells is due to Cell-Tak and not to any component such as fibronectin that is found in serum. This is seen in experiments involving the preincubation of Cell-Tak-coated dishes with either fibronectin or serum. Cell-Tak-coated plates were preincubated with cell culture medium containing 20% calf serum for 30 min, and fibronectin was dried onto other plates coated with Cell-Tak before U-937 cells were plated. Cell attachment on fibronectin-coated Cell-Tak plates was identical to attachment to Cell-Tak alone, and attachment efficiency was inhibited by 40% on plates preincubated with serum. 

Mutagenicity studies using Ames Salmonalla assays have been negative studies of sister chromatid exchange and mouse lymphoma line assays have been positive. 

Lazo PA, Klein-Szanto AJP, Tsichlis P N. T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus phenotypic diversity and its implications. J Virol 1990 64 3948-3959. 

The kinetics and extent of PDT-induced apoptosis also varies with photosensitizer and cell type treated. Photodynamic treatment of L5178Y murine lymphoma ceUs with ClAlPc resulted in apoptosis within 30 min of treatment. Treatment of the lymphoma line P388 with ClAlPc or tin etiopurpurin (SnEt ), which localize to the lysosome and the mitochondria, induced apoptotic nuclei within 1 However, treatment of the same cell line with tin octaethylpurpurin amidine, which localizes to the mitochondria, the lysosome, and the ceU membranes, did not result in apoptotic nuclei until 24 h after treatment. 

The multidrug resistance (mdr) reversing effect of the new phenothiazine complexes were tested on mouse T cell lymphoma cell lines. Trifluoperazine (TFP) was much more effective at the same concentration than verapamil. The efficacy of some metal coordination complexes [TFP-Cu(ll) and TFP-V(IV)] exceeded the action of TFP alone. Chlorpromazine (CPZ) or CPZ-Pt(ll) complex had the same or less effect than verapamil or promethazine (Pz) used as a control. 

As described in several monographs [4], bryostatin 1 exhibits significant in vitro and in vivo antineoplastic activity against a range of tumor cell lines including murine leukemia, B-cell lymphoma, reticulum cell sarcoma, ovarian carcinoma, and melanoma. It is also effective in the modulation of apoptotic function [5], the reversal of multidrug resistance [6], and stimulation of the immune system [7]. These unique features displayed by bryostatin 1 are attributed to its high affinity for protein kinase C (PKC) isozymes and its ability to selectively modulate their functions [8]. PKCs are a type of intracellular serine and threonine kinase that... 

Zevalin (Ibritumomab Tiuxetan murine monoclonal antibody, produced in a CHO cell line, targeted against the CD20 antigen) IDEC Pharmaceuticals Non-Hodgkin s lymphoma... 

The mouse lymphoma forward mutation test at the thymidine kinase locus (detects mutations to a nonfunctional thymidine kinase in a line of culture mouse lymphoma cells). 

Rituximab, a chimeric monoclonal antibody directed at the CD20 molecule on B cells, has become one of the most widely used therapies for follicular lymphoma. Rituximab is approved for first-line therapy either alone or combined with chemotherapy and as maintenance therapy for patients with stable disease or with partial or complete response following induction chemotherapy. 

Hainsworth, J.D. et al., Rituximab as first-line and maintenance therapy for patients with indolent non-Hodgkin s lymphoma, J. Clin. Oncol., 20,426, 2002. 

Joseph, D.B. and Bidlack, J.M., The kappa-opioid receptor expressed on the mouse lymphoma cell line Rl.l contains a sulfhydryl group at the binding site, Fur J. Pharmacol., 267, 1, 1994. 

The TK+/ fine was originally isolated as a spontaneously arising revertant clone from a UV-induced TK / clone. The parental TK+/+ cell and the heterozygote were then the only TK-competent mouse lymphoma cells that could be maintained in THMG medium (3 pg ml-1 thymidine, 5 pg ml-1 hypoxanthine, 0.1 pg ml-1 methotrexate and 7.5 pg ml-1 glycine) (Clive, 1987). Thus, like most established lines, these cells are remote from wild-type cells. The karyotype of the TK+/ —3.7.2C line has a modal chromosome number of 40 like wild-type, but has a variety of chromosomal rearrangements and centromeric heteromorphisms (Blazak et al., 1986). 

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