Bordetella pertussis

Pertussis toxin (from Bordetella pertussis) [70323-44-3J Mr 117,000. Purified by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and SDS-PAGE [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 79[Pg.557]

Pertussis toxin is produced by the bacterium Bordetella pertussis. It covalently modifies G-proteins of the G/Go family (transfer of a ADP-ribose moiety of NAD onto G-protein a-subunits). ADP-ribosylated G-proteins are arrested in their inactive state and, as a consequence, functionally uncoupled from their respective effectors. Examples for pertussis toxin-sensitive cellular responses include the hormonal inhibition of adenylyl cyclases, stimulation ofK+ channels, inhibition of Ca2+ channels and stimulation ofthe cGMP-phosphodiesterase in retinal rods. 

Bordetella pertussis is the causal organism of whooping-cough, a disease which... 

Killing. The proeess by which the live bacteria in the culture are killed and thus rendered harmless. Heat and disinfectants are employed. Heat and/or formalin are required to kill the cells of Bordetella pertussis used to make whooping-cough vaccines, and phenol is used to kill the Vibrio cholerae in cholera vaccine and the Salmonella typhi in typhoid vaeeine. 

Bacterial lipid A structures typically act as TLR2 agonists however, there are some cases where such compounds act as antagonists (e.g., Bordetella pertussis lipid A, 9), which antagonize the TLR2-TLR6 heterodimer [37]. 

Cytotoxin that inactivates G proteins involved in cellular metabolism. It is a solid obtained from bacteria (Bordetella pertussis). 

This material is hazardous through inhalation and penetration through broken skin. Specific signs and symptoms of exposure to this toxin have not been established or have not been published. However, the major symptoms associated with whooping cough, the disease caused by Bordetella pertussis, are related to the effects of this toxin. 

Bordetella pertussis Toxin Borer Sol Boroethane Boron Bromide Boron Chloride Boron Fluoride Boron Hydride Boron Tribromide Boron Trichloride Boron Trifluoride Botox... 

Bordetella pertussis Eubacteria 3.88 Arabidopsis thaliana Plant 70.0... 

It seems likely that many such (recombinant) subunit vaccines will gain future regulatory approval. One such example is that of Bordetella pertussis subunit vaccine. B. pertussis is a Gramnegative coccobacillus. It is transmitted by droplet infection and is the causative agent of the upper respiratory tract infection commonly termed whooping cough . 

Respiratory viruses are by far the most common infectious agents associated with acute bronchitis. The common cold viruses, rhinovirus and coronavirus, and lower respiratory tract pathogens, including influenza virus, adenovirus, and respiratory syncytial virus, account for the majority of cases. Mycoplasma pneumoniae also appears to be a frequent cause of acute bronchitis. Other bacterial causes include Chlamydia pneumoniae and Bordetella pertussis. 

Kajava, A. V., Cheng, N., Cleaver, R., Kessel, M., Simon, M. N., Willery, E., Jacob-Dubuisson, F., Locht, C., and Steven, A. C. (2001). Beta-helix model for the filamentous haemagglutinin adhesin of Bordetella pertussis and related bacterial secretory proteins. Mol. Microbiol. 42, 279-292. 

Oliver, D. C., Huang, G., Nodel, E., Pleasance, S., and Fernandez, R. C. (2003). A conserved region within the Bordetella pertussis autotransporter BrkA is necessary for folding of its passenger domain. Mol. Microbiol. 47, 1367-1383. 

The elegant and extensive studies of Szabo s group in Paris on LPS from Bordetella pertussis have been summarized in a plenary lecture that has appeared in print.71 From their studies, the authors concluded... 

Brennan, M. J., Hannah, J. H., and Leininger, E. (1991). Adhesion of Bordetella pertussis to sufatides and to the GalNAc-P-4Gal sequence found in glycosphingolipids. /. Biol. Chem. 266,18829-18831. 

Sandros, J., Rozdzinski, E., Zheng, J., Cowburn, D., and Tuomanen, E. (1994). Lectin domains in the toxin of Bordetella pertussis Selectin mimicry linked to microbial pathogenesis. Glycoconj.. 11,501-506. 

Examples of killed or inactivated vaccines are cholera vaccine containing dead strains of Vibrio cholerae, hepatitis A vaccine with inactivated hepatitis A virus, pertussis vaccine with killed strains of Bordetella pertussis, typhoid vaccine with killed Salmonella typhi, and influenza vaccine with various strains of inactivated influenza viruses (see Exhibit 4.2 for a discussion of influenza viruses and vaccines and Exhibit 4.3 on avian influenza H5N1). 

PT Bordetella pertussis AB4 GTPases Heterotrimeric Altered signal transduction Pertussis (whooping cough)... 

A nasopharyngeal swab obtained from a 4-month-old infrint with rhinitis and paroxysmal coughing tested positive upon culture for Bordetella pertussis. He was admitted to the hospital for therapy with an antibiotic that inhibits the translocation of peptidyl-tRNA on 70S ribosomes, This patient was most likely treated with... 

Pauwels, R., van der Straeten, M., Platteau, B., and Bazin, H. (1983) The non-specific enhancement of allergy. In vivo effects of Bordetella pertussis vaccine on IgE synthesis. Allergy. 38, 239-246. 

Pertussis toxin is a protein secreted by the bacterium Bordetella pertussis which causes whooping cough. The toxin enters a cell where it also catalyses ADP-ribosylation of the a-subunit of G-protein. 

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