Blue-Sepharose

Angiotensinogen (from human blood serum) [643I5-I6-8]. Purified by chromatography on Blue Sepharose, Phenyl-Sepharose, hydroxylapatite and immobilised 5-hydroxytryptamine [Campbell et al. Biochem J 243 121 1987]. 

Purified by ammonium sulfate pptn, then fractionated on Sephadex G-75 column, applied to a Blue Sepharose column and eluted with ImM dihydrofolate. [Al Rubeai and Dole Biochem J 235 301 1986.]... 

Pertussis toxin (from Bordetella pertussis) [70323-44-3J Mr 117,000. Purified by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and SDS-PAGE [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 79[Pg.557]

LC] and LC3, respectively. Similar LC] and LC3 aggregates were reported using blue-sepharose chromatography [27). 

Fisher, S.E. Whitt, G.S. Purification of the creatine kinase isozymes of the green sunfish (Lepomis cyanellus) with Blue Sepharose CL-6B. Anal. Biochem., 94, 89-95 (1979)... 

Knowles, P. P. and Thorpe, P. E. (1987) Purification of immunotoxins containing ricin A chain and abrin A chain using Blue Sepharose CL-6B. Anal Biochem. 160,440-443. 

The small amounts of the All desaturase available make solubilization and stabilization necessary before purification can be attempted. A series of detergents previously reported useful in desaturase purifications proved not to be useful in this case -indeed, most of them actually lowered the activity. However, octanoyl-N-methyl glucamide (21) did appear to stabilize and at least partially solubilize the enzyme. Treatment of enzyme solutions with Blue Sepharose, an affinity chromatography... 

Cibacron Blue is a blue, polyaromatic, sulfonated dye (Fig. 6). It can be attached, as an affinity li nd, to solid matrix supjmrts (e.g. dextran, agarose) by the reaction of the triazine ring with free hydroxyl groups of the supports. The conditions of this triazine coupling method have been described by Bohme Such a dye affinity sorbent is also produced commercially, e.g. under trade name Blue Sepharose CL-6B (Cibacron Blue F3G-A covalently bound to the cross-linked agarose gel Sepharose CL-6B ) by Pharmacia, Sweden. 

Blue Sepharose.CL-6B, firm literature of Pharmacia, Uppsala, Sweden Haff, L. A., Easterday, R. L. Cibacron Blue-Sepharose A tool for general ligand affinity chromatography, in Theory and Practice in Affinity Technoques, (ed. Sundaram, P. V., Eckstein, F.) Academic Press 1978... 

Anion-exchange HPLC (Mono Q HR 5/5 column) and affinity chromatography (Hi-Trap Heparin and Hi-Trap Blue-Sepharose columns) and IDA-ICP-MS with an octapole reaction system ( Se/ Se and °Sef Se)... 

Angiotensinogen (from procine plasma) [64315-16-8] Mr 59,400, 60,000, 62,600, 63,600 depending on sialic acid content. This rennin substrate is purified 390-fold from the serum by chromatography on Blue Sepharose, phenyl sepharose, hydroxyapatite and finally by affinity chromatography on... 

From Pharmacia (Piscataway, NJ) Protein A-Sepharose Fast Flow, Protein G-Sepharose Fast Flow, Sephacryl S-200HR, DEAE-Sepharose CL-4B, Sephadex G-25M, Blue-Sepharose CL-4B, Sephadex G-25 MicroSpin, CM-Sepharose CL-4B, SP-Sepharose Fast Flow. 

The thiolated dgRTA is loaded onto a Blue-Sepharose CL-4B column equilibrated with borate-saline buffer. 

The concentrations of both IgG-MPT and dgRTA-SH are brought to 3-5 mg/mL (after filtration through a 0.22 pm filter) and the mixture is incubated for 24-48 h at 25°C. The purification involves removing the unreacted IgG by affinity chromatography on Blue-Sepharose CL-4B equilibrated with PBE. 

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