Biosensors gold nanoparticle

Key words Electrochemical biosensors, Gold nanoparticles, Enzyme biosensors, Immunosensors, Composite electrodes, Carbon nanotubes. 

The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticles and nanotubes nanomaterial-based electrochemical DNA detection electrochemical detection of microorganism-based DNA biosensor gold nanoparticle-based electrochemical DNA biosensors electrochemical detection of the aptamer-target interaction nanoparticle-induced catalysis for DNA biosensing basic terms regarding electrochemical DNA (nucleic acids) biosensors screen-printed electrodes for electrochemical DNA detection application of field-effect transistors to label-free electrical DNA biosensor arrays and electrochemical detection of nucleic acids using branched DNA amplifiers. 

In 1994, thiols were firstly used as stabilizers of gold nanoparticles [6a]. Thiols form monolayer on gold surface [18] and highly stable nanoparticles could be obtained. Purification of nanoparticles can be carried out, which makes chemical method of metal nanoparticles a real process for nanomaterial preparation. Various thiol derivatives have been used to functionalize metal nanoparticles [6b, 19]. Cationic and anionic thiol compounds were used to obtain hydrosols of metal nanoparticles. Quaternary ammonium-thiol compounds make the nanoparticle surface highly positively charged [20]. In such cases, cationic nanoparticles were densely adsorbed onto oppositely charged surfaces. DNA or other biomolecule-attached gold nanoparticles have been proposed for biosensors [21]. 

Zhong X, Yuan R, Chai Y, Liu Y, Dai J, Tang D (2005) Glucose biosensor based on self-assembled gold nanoparticles and double-layer 2d-network (3-mercaptopropyl)-trimethoxy-silane polymer onto gold substrate. Sensor Actuator B 104 191-198... 

Zhao W, Chiuman W, Lam JCF, McManus SA, Chen W, Cui Y, Pelton R, Brook MA, Li Y (2008) DNA aptamer folding on gold nanoparticles from colloid chemistry to biosensors. J Am Chem Soc 130(11) 3610-3618... 

Hu, Y., et al., Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing. Biosensors and Bioelectronics,... 

V. Carralero Sanz, M.L. Mena, A. Gonzalez-Cortes, P. Yanez-Sedeno and J.M. Pingarron, Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes. Application to the measurement of a bioelectrochemical polyphenols index in wines, Anal. Chim. Acta, 528(1) (2005) 1-8. 

Recently, a renewable potentiometric urease inhibition biosensor based on self-assembled gold nanoparticles has been developed by Yunhui et al. for the determination of mercury ions [43]. The advantages of self-assembled immobilization are low detection limit (0.05 iM), fast response, and relatively easy regeneration of the biosensors. The assembled gold nanoparticles and inactivated enzyme layers denatured by Hg2+ can be rinsed out via a saline solution with acid and alkali successively. 

Y. Yang, Z. Wang, M. Yang, M. Guo, Z. Wu, G. Shen and R. Yu, Inhibitive determination of mercury ion using a renewable urease biosensor based on self-assembled gold nanoparticles, Sens. Actuators B, 114 (2006) 1-8. 

Pingarron, J.M., P. Yanez-Sedeno, and A. Gonzalez-Cortes. 2008. Gold nanoparticle-based electrochemical biosensors. Electrochim. Acta 53 5848-5866. 

Yang W, Li Y, Bai Y, Sun C (2006) Hydrogen peroxide biosensor based on myoglobin/ colloidal gold nanoparticles immobilized on glassy carbon electrode by a Nafion film. Sens Actuators B 115 42—48... 

Cui RJ, Huang HP, Yin ZZ, Gao D, Zhu JJ (2008) Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor. Biosens Bioelectron 23 1666-1673... 

Wang TJ, Lin CS (2006) Electro-optically modulated localized surface plasmon resonance biosensors with gold nanoparticles. Appl Phys Lett 89 173903... 

Wu, H.-T., Chen, Y.-L., Huang, Y.-C., et al. (2007) Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor. Proceedings ofSPIE 6447 64470S-1 —64470S-7. 

Hsieh B.-Y., Chang Y.-F., Ng M.-Y., Liu W.-C., Lin C.-H., Wu H.-T., and Chou C. (2007). Localized surface plasmon coupled fluorescence fiber-optic biosensor with gold nanoparticles. Anal. Chem. 79, 3487-3493. 

Biosensors Based on Proteins Immobilized on Gold Nanoparticles... 

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