Fiber optic biosensors

Fluororescently labeled toxin captured and immobilized on the surface of the optical waveguide GMl 20 minutes Magnetoelastic immunosensor Piezoelectric immunosensor Wavelength modulation-based SPR biosensor Fiber optic surface plasmon resonance ... 

The dye is excited by light suppHed through the optical fiber (see Fiber optics), and its fluorescence monitored, also via the optical fiber. Because molecular oxygen, O2, quenches the fluorescence of the dyes employed, the iatensity of the fluorescence is related to the concentration of O2 at the surface of the optical fiber. Any glucose present ia the test solution reduces the local O2 concentration because of the immobilized enzyme resulting ia an iacrease ia fluorescence iatensity. This biosensor has a detection limit for glucose of approximately 100 ]lM , response times are on the order of a miaute. 

FIBER OPTIC CHEMICAL SENSORS AND BIOSENSORS A VIEW BACK... 

Moreno-Bondi 1990 fiber optic glucose biosensor (via oxygen)... 

Fiber-optic biosensors based on luminescence and immobilized enzymes for the detection of NADH and ATP can be found in ref. (147-152). 

While several optical chemical sensors and biosensors in use that do not rely on fiber optics, the commercialization of FOCS technology started slowly, probably because of the limitations imparted to optical sensing if... 

Wolfbeis O.S. (ed.), Fiber Optic Chemical Sensors and Biosensors, CRC Press, Boca Raton, Florida, 1991. 

Barker S.L., Kopelman R., Meyer T.E., Cusanovich M.A., Fiber-Optic Nitric Oxide-Selective Biosensors, Anal. Chem. 1998 70 971. 

Schaffar B.P.H., Wolfbeis O.S., Chemically Mediated Fiber Optic Biosensors, chapter 8 in Biosensors Principles and Applications, L.J. Blum, P.R. Coulet (eds.), M. Dekker, New York, chapter 8, pp. 163-194 (1991). 

Marazuela M.D., Moreno-Bondi M.C., Fiber-optic biosensors - an overview, Anal. Bioanal. Chem. 2002 372 664. 

Dremel B.A., Schaffar B.P., Schmid R.D., Determination of glucose in wine and fruit juice based on a fiber-optic glucose biosensor and flow-injection analysis, Anal. Chim. Acta 1989 225 293. 

Moreno-Bondi M.C., Wolfbeis O.S., Leiner M.J.P., Schaffar B.P.H., Oxygen optrode for use in a fiber optic glucose biosensor, Anal. Chem. 1990 62 2377. 

Trettnak W., Wolfbeis O.S., Fiber optic cholesterol biosensor with an oxygen optrode as the transducer Anal. Biochem. 1990 184 124. 

W. Trettnak, O.S. Wolfbeis, A fully reversible fiber optic lactate biosensor based on the intrinsic fluorescence of lactate monooxygenase, Fresenius Z. Anal. Chem. 1989, 334, 427. 

Freeman M.K., Bachas L., Fiber-optic biosensor with fluorescence detection based on immobilized alkaline phosphatase, Biosensors Bioelectron. 1992 7 49. 

Scheper T., Bueckmann A.F., A fiber optic biosensor based on fluorometric detection using confined macromolecular nicotinamide adenine dinucleotide derivatives, Biosens. Bioelectron. 1990 5 125. 

Gautier S.M., Blum L.J., Coulet P.R., Fiber-optic sensor with Co-immobilized bacterial bioluminescence enzymes, Biosensors 1989 4 181. 

Zhou X., Arnold M.A., Internal enzyme fiber-optic biosensors for hydrogen peroxide and glucose, Anal. Chim. Acta 1995 304 147-156. 

Blum L.J., Chemiluminescent flow injection analysis of glucose in drinks with a bienzyme fiber optic biosensor, Enzyme Microb. Technol. 1993 15 407-411. 

Hlavay J., Guilbault G.G., Determination of sulphite hy use of a fiber-optic biosensor based on a chemiluminescent reaction, Anal. Chim. Acta 1994 299 91-96. 

Zang W., Chang H., Rechnitz A., Dual enzyme fiber-optic biosensor for pyruvate, Anal. Chim. Acta. 1997 350 59-65. 

Walters B.S., Nielsen T.J., Arnold M.A., Fiber-optic biosensor for ethanol based on an internal enzyme concept, Talanta 1988 35 151-155. 

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