Urease inhibition

Wittekind E, Werner M, Reinicke A, Herbert A, Hansen P (1996) A microtiter-plate urease inhibition assay-sensitive rapid and cost-effective screening for heavy metals in water. Environ Technol 17 597-603... 

Urease inhibition. Water extract of the seed, in cell culture at a concentration of 0.3 mg/mL, was inactive . 

Turgal stimulant activity. Hot water extract of 7 g Bupleurum falcatum, 5 g Pinelhi temata, 3 g Scutellaria baicalensis, 4 g Zin ber officinale, 3 g Panax ginseng, 3 g Zizyphus inermis, and 2 g Glycyrrhiza glabra, administered intraperitoneally to rats at a dose of 200 mg/kg, decreased the volume of exudate in carrageenan-induced pleurisy ° k Urease inhibition. Water extract of the rhizome, at a concentration of 0.3 mg/mL, was inactive ° k... 

Recently, a renewable potentiometric urease inhibition biosensor based on self-assembled gold nanoparticles has been developed by Yunhui et al. for the determination of mercury ions [43]. The advantages of self-assembled immobilization are low detection limit (0.05 iM), fast response, and relatively easy regeneration of the biosensors. The assembled gold nanoparticles and inactivated enzyme layers denatured by Hg2+ can be rinsed out via a saline solution with acid and alkali successively. 

If you want to perform the urease inhibition experiment but you cannot obtain HgCl2, what could you substitute as an enzyme inhibitor ... 

An alternative scenario was put forward based on the crystal structures of urease inhibited by either phosphate, diamidophosphate, or borate (4,5, 28). It gets some support from the kinetic findings for fluoride inhibition of urease (29), as well as from recent model calculations (30, 31). Boric acid, known to be a competitive inhibitor of urease, can be considered a good substrate analogue, since it is isoelectronic with urea and has the same shape and dimension. Bacillus pasteurii could be crystallized in the presence of boric acid. The structure reveals that a molecule of B(OH)3 is symmetrically spanning the nickel ions, replacing Wj, W2,... 

Znllo, A., Rinaldi, V., Hassan, C., Folino, S., Winn, S., Knto, G., Attili, AJ . Helicobacter pyloric and plasma ammonia levels in cirrhotics role of urease inhibition by acetohydroxamic acid. Ital. J. Gastroenterol. Hepatol. 1998 30 405-409... 

Brack W, Paschke A., Segner H., Wennrich R., and Schiirmann G., Urease inhibition A tool for toxicity identification in sediment elutriates, Chemosphere, 40, 829-834, 2000. 

M D Sweidloff, J F Kolc, M M Rogic and L L Hmbickstni, Phosphoroamide urease inhibitors and urease inhibited urea based fertilizer compositions, US Patent Office, Pat No... 

Benini S, Rypniewski WR, Wilson KS, Ciurli S, Mangani S (2001) Structure-based rationalization of urease inhibition by phosphate novel insights into the enzyme mechanism. J Biol Inorg Chem 6(8) 778-790. doi 10.1007/s007750100254... 

Pantothenic Chick Urease Inhibition McCoy and Nair, 1954... 

CERVELLI S., NANNIPIERI P., GIOVANNINI G. and PERNA A. 1977. Effect of soil on urease inhibition by substituted urea herbicides. Soil Biology and Biochemistry, 9, 393-396. 

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