Horseradish peroxidase function

Cui RJ, Huang HP, Yin ZZ, Gao D, Zhu JJ (2008) Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor. Biosens Bioelectron 23 1666-1673... 

GNP/CNT hybrid platform with horseradish peroxidase-functionalized GNP label for sensitive detection of human IgG as a model protein This approach provided a linear response range between 0.125 and 80 ng/mL, with a detection limit of 40 pg/mL and showed good precision, with acceptable stability and reproducibility. Used for the detectirai of human IgG in real samples, which provides a potential alternative tool for the detection of protein in the clinical laboratory Cui et al. [60]... 

Song, Z., R. Yuan, Y. Chai et al. 2010. Horseradish peroxidase-functionalized Pt hollow nanospheres and multiple redox probes as trace labels for a sensitive simultaneous multianalyte electrochemical immunoassay. Chem. Commun. 6750-2675. 

It should be pointed out that the addition of substances, which could improve the biocompatibility of sol-gel processing and the functional characteristics of the silica matrix, is practiced rather widely. Polyethylene glycol) is one of such additives [110— 113]. Enzyme stabilization was favored by formation of polyelectrolyte complexes with polymers. For example, an increase in the lactate oxidase and glycolate oxidase activity and lifetime took place when they were combined with poly(N-vinylimida-zole) and poly(ethyleneimine), respectively, prior to their immobilization [87,114]. To improve the functional efficiency of entrapped horseradish peroxidase, a graft copolymer of polyvinylimidazole and polyvinylpyridine was added [115,116]. As shown in Refs. [117,118], the denaturation of calcium-binding proteins, cod III parvalbumin and oncomodulin, in the course of sol-gel processing could be decreased by complexation with calcium cations. 

Horak, D., Karpisek, M., Turkova, J., and Benes, M. (1999) Hydrazide-functionalized poly(2-hydroxye-thyl methacrylate) microspheres for immobilization of horseradish Peroxidase. Biotechnol. prog. 15, 208-215. 

L. Alfonta, I. Willner, D.J. Throckmorton, and A.K. Singh, Electrochemical and quartz crystal microbalance detection of die cholera toxin employing horseradish peroxidase and GM1-functionalized liposomes. Anal. Chem. 73, 5287—5295 (2001). 

Advances have been achieved in recent years, such as the use of CL reagents as labels to derivatize and sensitively determine analytes containing amine, carboxyl, hydroxy, thiol, and other functional groups and their application in HPLC and CE [35, 36], the synthesis and application of new acridinium esters [37], the development of enhanced CL detection of horseradish peroxidase (HRP) labels [38], the use of immobilization techniques for developing CL-based sensors [39-42], some developments of luminol-based CL in relation to its application to time-resolved or solid-surface analysis [43], and the analytical application of electrogenerated CL (ECL) [44-47], among others. 

Horseradish peroxidase (HRP) is an extracellular plant enzyme that acts in regulation of cell growth and differentiation, polymerization of cell wall components, and the oxidation of secondary metabolites essential for important pathogenic defense reactions. Because of these essential functions, and also because of its stability and ready availability, HRP has attracted considerable attention.13 It has been involved in a number of applications, such as diagnostic assays,14 biosensors,15 bioremediation,16 polymer synthesis,17 and other biotechnological processes.18 More applications in which HRP catalysis is translated into an electrochemical signal are likely to be developed in the near future. 

Alvarez S, Manolache S, Denes F (2003) Synthesis of polyaniline using horseradish peroxidase immobilized on plasma-functionalized polyethylene surfaces as initiator. J Appl Polym Sci 88(2) 369-379... 

This supposition is supported by the correlation of the rate constants for product formation from metMb, horseradish peroxidase, Cu, Zn SOD, Mn SOD, and GSH (147). Electrophilic addition of HNO is assumed to be the rate-limiting step in these reactions. In comparison, the addition of HNO to NH2OH is substantially slower under neutral conditions 1 01 M 1 s 1 (148)], which may be a function of amine pK.r... 

As expected, the assimilation of horseradish peroxidase by generalized gangliosidosis fibroblasts was found to be a linear function of the concentration of the enzyme in the growth medium (Figure 4). 

Morawski B, Lin Z, Cirino P et al (2000) Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng 13 377-384... 

Morawski B, Quan S, Arnold FH (2001) Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae. Biotechnol Bioeng 76 99-107... 

Mogharrab N, Ghourchian H, Amininasab M (2007) Structural stabilization and functional improvement of horseradish peroxidase upon modification of accessible lysines experiments and simulation. Biophys J 92 1192-1203... 

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