Fiber-optic internal enzyme biosensor

The first example of a fiber-optic internal enzyme biosensor has been reported for the determination of ethanol (13). A microporous Teflon membrane is used as the perm-selective membrane for this ethanol sensor. Alcohol dehydrogenase is the enzyme and the following reactions take place in the sensor ... 

Instead, the enzyme is separated from the sample by a perm-selective membrane that allows the analyte of interest to enter an internal solution. This internal solution contains all the reagents required for the analytical reaction, including the enzyme. Figure 9 is a schematic diagram of an internal enzyme biosensor with a fiber-optic detection scheme. As the sample enters the enzyme-containing internal solution, the analytical reaction takes place. The rate of this reaction is monitored and related to the concentration of the analyte in the sample solution. 

Zhou X., Arnold M.A., Internal enzyme fiber-optic biosensors for hydrogen peroxide and glucose, Anal. Chim. Acta 1995 304 147-156. 

Walters B.S., Nielsen T.J., Arnold M.A., Fiber-optic biosensor for ethanol based on an internal enzyme concept, Talanta 1988 35 151-155. 

Walters, B. S., Nielson, T. J. Arnold, M. A., Fiber-Optic Biosensors for Ethanol, Based on an Internal Enzyme Concept , Talanta 35 (1988) 151-156. 

Figure 9. Schematic diagram for the internal enzyme fiber-optic biosensor.

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