Biomaterials adhesive proteins

Barrias CC, Martins MCL, Almeida-Porada G, Barbosa MA, Granja PL (2009) The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers. Biomaterials 30 307-316... 

Cell adhesion, protein-mediated, on biomaterials, 143-145 Ceramics... 

These studies provide a base for further development of collagen materials for specific biomaterial applications. Further studies are being directed to the effect of collagen film on blood in terms of platelet and white cell adhesion, protein absorption, and thrombogenicity. 

Chen YM, Shiraishi N, Satokawa H. Kakugo A. Narita T, Gong JP, Osada Y, Yamamoto K, Ando J (2005) Cultivation of endothelial ceUs on adhesive protein free synthetic polymer gels. Biomaterials 26 4588-4596... 

Proteins play a critical role in regulating cell interactions with both biological and synthetic surfaces. The type and density of proteins presented on a surface are major determinants in cell function. Because proteins are present in all bodily fluids and most cell culture media, it is important to understand the dynamics of protein adsorption to biomaterials. Additionally, protein adsorption to materials impacts the overall performance of biomaterials in several manners, including regulation or inhibition of cell adhesion. 

Hydrophobicity of biomedical polymers influences the biocompatibility depending on the particular application such as tissue engineering, blood contacting devices, and dental implants [35]. Polymers are dynamic structures and can switch their surface functional groups depending on the environment. For example, polymeric biomaterials need to have a hydrophilic smface for most of the applications, so that the cell-adhesive proteins present in the serum will be adsorb and promote cell adhesion and proliferation. This is achieved by snrface treatment procedures such as... 

Hwang, D.S., Sim, S.B., Cha, H.J., 2007b. Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide. Biomaterials 28, 4039—4046. 

Hwang, D.S., Waite, J.H., Tirrell, M., 2010. Promotion of osteoblast proliferation on complex coacervation-based hyaluronic acid — recombinant mussel adhesive protein coatings on titanium. Biomaterials 31, 1080—1084. 

After a biomaterial is implanted in the body, it takes seconds to minutes for proteins to adsorb and cover its surface, forming the so-called conditioning film [11], Therefore, instead of the original surface of the implanted material, the cells will recognize and interact with this protein layer. It is fair to assume that the adhesion proteins are responsible for converting the biomaterials into biologically recognizable materials. The adsorption of these adhesion proteins is the basis for all the reactions that may further occur in the body [33]. 

The surface properties of the biomaterials will determine the type, amount and conformation of the adsorbed proteins [2]. The composition of this protein layer can be different, depending on the fluid composition and adsorption time [25], Besides the composition of the protein layer, the conformation and the orientation of the protein can also change with time [8]. This conditioning protein layer will increase the cell adhesiveness, since the cells have receptors in their membranes that specifically bind to the adhesion proteins. Moreover, the protein layer also increases the cell spreading at the biomaterial surface [6,10]. 

Tziampazis E, Kohn J, Moghe PV (2000) PEG-variant biomaterials as selectively adhesive protein templates model surfaces for controlled cell adhesion and migration. Biomateiials 21 511-520... 

The chemical and topographical characteristics of surfaces have profoimd effects on cellular, tissue, and host responses to synthetic materials [11, 31]. Consequently, surface modifications of chemistry and roughness have been introduced to improve performance in virtually all materials used in biotechnological [e.g., tissue culture and enzyme-linked immimosorbent assay (ELISA) plates, gene and protein array chips, bioseparation and bioprocess matrices] and biomedical (e.g., vascular grafts, orthopedic and dental implants, biosensors, catheters) appUcations. This review focuses on interfaces controlling cell-biomaterial adhesive interactions via manipulations of material surface chemistry to modulate protein adsorption and activity. 

Metallothionein. See also http //www.bioc.uzh.ch/mtpage/MT.html http / /www.ithyroid. com / metallothionein.htm http //www.bioscience.org/knockout/mt-land2.htm http //biomaterials.bme.northwestern.edu/mussel.asp Mussel Adhesive Protein Mimetics. Website from Phillip B. 

Biomaterials with Low Thrombogenicity. Poly(ethylene oxide) exhibits extraordinary inertness toward most proteins and biological macromolecules. The polymer is therefore used in bulk and surface modification of biomaterials to develop antithrombogenic surfaces for blood contacting materials. Such modified surfaces result in reduced concentrations of ceU adhesion and protein adsorption when compared to the nonmodifted surfaces. 

---