Biomaterial self-assembled monolayers

Arima Y, Iwata H (2007) Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials 28 3074-3082... 

Faucheux N, Schweiss R, Liitzow K, Werner C, Groth T (2004) Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies. Biomaterials... 

Barrias CC, Martins MCL, Almeida-Porada G, Barbosa MA, Granja PL (2009) The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers. Biomaterials 30 307-316... 

Pakalns, T., Haverstick, K. L., Fields, G. B., et al. Cellular recognition of synthetic peptide amphiphiles in self-assembled monolayer films. Biomaterials 20 2265—2275,... 

Humblot V, Yala JF, Thebault P et al. (2009) The antibacterial activity of Magainin I immobilized onto mixed thiols self-assembled monolayers. Biomaterials 30 3503-3512... 

Oxide surfaces can be functionalized using a silane-based self-assembled monolayer. Amine functional groups are commonly utilized for functionalizing surfaces with biomaterials, by forming amide bonds. A common method is to expose the surface to 0.1 M aminopropyltri-methoxysilane (APTS) in toluene at 100 °C for 10 min to 1 h. This treatment renders the surface linkable with many biomaterials with a carboxylic group (-COOH). Various silanes can be utilized to decorate the surface with desired functional groups. Readers are referred to the article methods for surface modification for further understanding. 

This chapter deals primarily with monolayers of surfactants at fluid interfaces, but some attention is also given to (nano) coatings such as Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) films, self-assembled monolayers (SAMs), and layers obtained by alternating polyelectrolyte deposition. Such coatings may be applied for the functionalization of surfaces, for instance, to achieve biocompatibility of biomaterials, improve specificity and selectivity of biosensors and membranes, and control immobilization of enzymes or cells in bioreactors. 

Inaba, R. Khademhosseini, A. Suzuki, H. Fukuda, J. Elecirochemical desorption of self-assembled monolayers for engineering ceUular tissues. Biomaterials 2009,30,3573-3579. 

Lan MA, Gershach CA, Michael KE, Keselowsky BG, Garcia AJ. Myoblast prohfer-ation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries. Biomaterials 2005 26 4523-31. 

The use of organic solvents to produce surface layers has clear disadvantages if the aim is to use such techniques on an industrial scale (due to both environmental emission and disposal issues). Moreover, for applications in areas such as medical devices and implants, the presence of even minor organic solvent residues in the adlayers cannot be tolerated, in view of potential cell-toxicity effects and other biological risks. On the other hand, self-assembled monolayers, in particular of molecules with functional terminal groups, are of great interest for the modification and functionalization of biomaterials and medical devices. Therefore, a technique based on the deposition of SAMs from aqueous alkyl phosphate solutions has been developed and successfully applied to a variety of metal oxide substrates. ... 

Self-assembled monolayers (SAMs) of alkanethiolates on metal surfaces constitute a class of molecular assemblies formed by the spontaneous chemisorption of long-chain functionalized molecules on the surface of solid substrates. Due to their ease of preparation, long-term stability, controllable surface chemical functionality, and high, crystal-like, two-dimensional order, SAMs represent suitable model surfaces to study molecular adsorption, adhesion, wetting, lubrication, and the interaction of proteins and cells with artificial organic surfaces. The latter phenomena are of crucial importance to the fields of biomaterials, biosensors, and medical devices. 

Raynor, J. E., Capadona, J. R., CoUard, D. M., Petrie, T. A., and Garcia A. J. 2009. Polymer brushes and self-assembled monolayers versatile platforms to control cell adhesion to biomaterials. Biointerphases 4 FA3-FA16. 

Murugan, R., P. Molnar et al. 2009. Biomaterial surface patterning of self assembled monolayers for controlling neuronal cell behavior. International Journal of Biomedical Enginnering Technology 2(2) 104-134. 

Chung YC, Chiu YH, Wu YW, Tao YT (2005) Self-assembled biomimetic monolayers using phospholipid-containing disulfides. Biomaterials 26 2313-2324... 

The self-assembly and inhibition of protein adsorption by thiolated dextran monolayers at hydrophobic metal surfaces. In Ratner BD, Castner DG (eds) Surface modification of polymeric biomaterials. Plenum, New York, p 117... 

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