Bioassays Ames test

Chemical Rat Bioassay Mouse Bioassay Ames Test Enslein s Model... 

Ames test analy chem A bioassay that uses a set of histidine auxotrophic mutants of Salmomlla typhimurium for detecting mutagenic and possibly carcinogenic compounds. amz, test ... 

Muta-Chromoplate is a modified version of the classical Ames test for the evaluation of mutagenicity. The bioassay uses a mutant strain of S. typhimurium. The reverse mutation is recorded as absence of bacterial growth after 5 days incubation [55]. 

Several short-term bioassay procedures (3-14) have been developed recently which are applicable to detecting mutagenic and potential carcinogenic activity of organic substances. The SaimoneJla/mammalian mlcrosome assay or Ames Test (13-13] has been the most frequently applied and its efficacy has been well documented. This assay has also been applied to complex mixtures (19-22) to reduce greatly the time... 

LOPER I have a question for Cotruvo. I think that one thing strictly from the bioassay point of view (and that comes down to being 98 Ames test data) is that there is a logical approach from the practical point of view of using Ames test data. 

Two bioassays a) 48h plate incorporation AMES test with Salmonella typhimurium his- (TA 98, 100, 1535, 1537 et 1538) b) 24h acute crustacean test (Daphnia magna) MSW landfill leachates Ashes and slags from MSW incinerator leachates Centrifuged and 0.45 pm filtered only for AMES test... 

Four bioassays a) 30 min acute bacterial test (Vibrio fischeri) b) 72h algal test (Pseudokirchneriella subcapitata) c) Acute (24h) and chronic (28d) crustacean test (Daphnia magna) d) 48h plate incorporation AMES test (Salmonella typhimurium his-with TA 97a, 98, 100, 102) Industrial solid waste leachates Batch leaching test with demineralized water followed by paper filtration (crude leachate), Liquid/liquid extraction (organic extract), lyophilization (lyophilized extract. pH adjusted according to tolerance of organisms and 0.22 pm filtration for AMES test... 

With respect to leachate pre-treatment, different methods have been reported in the literature involving steps such as decantation, centrifugation, filtration and pH adjustment. Specific pre-treatment may well have some influence on chemical or ecotoxicological results (Isidori et ah, 2003). Moreover, some bioassays can only be run on 0.45 pm filtered leachates (e.g. Ames test) or those having undergone pH adjustment (e.g., Microtox test). 

The first step in the tier system of genetic toxicology blind bioassay testing is the Salmonella, microbial (Ames) test. 

Results from one short-term feeding study indicate patchouly oil to be nontoxic to rats. The flavonoids isolated via bioassay-guided fractionation from patchouli displayed in vitro antimutagenic activity against furylfuramide and Trp-P-1 in the Ames test at less than 1 xM levels. ... 

Brusick D. 1983. Evaluation of chronic rodent bioassays and Ames assay tests as accurate models for predicting human carcinogens. In Milman HA, ed. Application of biological markers for carcinogen testing, 153-163. 

Sodium borate tested negatively in the Ames bioassay but was found to be cytotoxic to cultured human fibroblasts."... 

As with the Salmonella reversion assay, this shortterm test is conducted both without (— PMS) and with metabolic activation produced by addition of post-mitochondrial supernatant containing rat liver enzymes ( + PMS). These terms are equivalent to — S9 and + S9 in the Ames reversion assay we use the latter designation for both types of bacterial assays. A more sensitive micro-forward mutation bioassay using this TM677 strain to determine the mutagenicity of indoor air particles, including ETS and wood smoke, is described by Lewtas et al. (1987). 

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