Bio-Rad Laboratories

KnowItAll Bio-Rad s SadtlerT software database solutions for spectroscopy numeric IR, NMR, MS, NIR, and Raman data Bio-Rad Laboratories, Inc. commercial CD-ROM periodi- cally www.bio-rad.- com... 

H.F. Schaefer III, P. R. Schreiner (Eds.), John Wiley Sons, Chichester, 1998, pp. 1845-1857. pq ACD/lLab - Interactive Laboratory, http //wwiv.acdlab5.com/ilab p7] Chemical Concepts (Whey-VCH), http //www.chemicalconcepts.com p ] Bio-Rad Laboratories, Inc., kttp //wmv.bio-rad.com P9] M. Tusar, L. Tusar, S. Bohanec,... 

Bio-Rex is the trade name for certain resins sold by Bio-Rad Laboratories. 

Standard Proton NMR Collection, Sadder Research Laboratories, Division of Bio-Rad Laboratories, Inc., Philadelphia, Pa., 1980. 

The checkers used beads of chloromethylated polymer available from Bio. Rad. Laboratories, Richmond, California (Bio Beads S-X2). Chlorine analysis (Note 3) showed that the resin contained 1.06 milli-equivalents of chlorine per gram, as specified by the manufacturer. 

Bio-Beads S-X3, 200-400 mesh (Bio-Rad Laboratories, Munich, Germany) Cyclohexane, for residue analysis Ethyl acetate, for residue analysis... 

Solid-phase extraction (SPE) cartridge, LC Si 2-g, 12-mL (Supelco), or equivalent ABC Laboratories Model SP 1000 gel permeation chromatograph system equipped with a 2.5 x 32.0 cm glass column of Bio-Beads S-X3 Select 200-400 mesh (ca 50 g, Bio-Rad Laboratories) preconditioned with ethyl acetate-cyclohexane (1 1, v/v), or equivalent... 

Many instrumentation companies have commercialized complete or modular CE systems. The principal vendors are Beckman Instruments, Inc. (Fullerton, CA) Bio-Rad Laboratories (Hercules, CA) Dionex Co. (Sunnyvale, CA) Hewlett Packard (Palo Alto, CA) ISCO, Inc. (Lincoln, NE) Otsuka, Ltd. (Tokyo, Japan) Perkin-Elmer -ABI (Foster City, CA) Thermo-Separation Products (Fremont, CA) and Waters Associates (Milford, MA). 

HPLC analysis for succinic acid, succinamic acid, succinamide, succinimide, N-methylsuccinimide, butyric acid, and propionic acid was performed with a Waters Model 515 HPLC pump equipped with a Waters Model 2410 Refractive Index Detector. Separations were performed with an Aminex HPX-87H 300mm column (Bio-Rad Laboratories, Hercules, CA) operated at 35°C, and using 0.005 M H2S04 elluent. 

Spoly(vinylbenzylchloride). -Cross-linked using divinylbenzene. Chloromethylated, cross-linked polystyrene resins were obtained coiranercielly from Bio-Rad Laboratories. Percent chloromethylation js based on the available phenyl groups in the polymer that is minus the percent cross-linking. =D=dioxane E ethanol. Percent of available chloromethyl croups reacted with donor. —Percent reaction x percent chloromethylation. Polymer prepared by free-radical polymerization of 60.00 para-neta chloromethylated sytrene (Dow Chemical). Reaction heated at 50-55°C. 

Tim Wehr is Staff Scientist at Bio-Rad Laboratories in Hercules, California. He has more than 20 years of experience in biomolecule separations, including development of HPLC and capillary electrophoresis methods and instrumentation for separation of proteins, peptides, amino acids, and nucleic acids. He has also worked on development and validation of LC-MS methods for small molecules and biopharmaceuticals. He holds a B.S. degree from Whitman College, Walla Walla, Washington, and earned his Ph.D. from Oregon State University in Corvallis. 

Stephen Tuck Dynavax Technologies, Berkeley, California Martin Vanderlaan Genentech, Inc., South San Francisco, California Tim Wehr Bio-Rad Laboratories, Hercules, California Ping Wong Genentech, Inc., South San Francisco, California Mingde Zhu Bio-Rad Laboratories, Hercules, California... 

CSEARCH/HaveltAlF NMR, Bio-Rad Laboratories, Informatics/Sadtler Group, 3316 Spring Garden Street, Philadelphia, PA 19104-2596, USA. 

Bio-beads consists of (1% cross-linked polystyrene with 1.25 mmol chloromethyl substitution per gram of dry resin respectively benzhydrylamine polymer (1% cross-linked polystyrene with 0.24 mmol NH2 per gram of dry resin, Bio-Rad Laboratories (Richmond, CA, USA). 

Gel Permeation Chromatography. Samples were filtered on columns of Bio-Gel P-6DG (Bio-Rad Laboratories), and columns of Sephadex G-10, G-25, and G-50 (Pharmacia Corp.) using deionized water as eluant. Gel filtration properties are expressed in terms of the distribution coefficient calculated from the relationship = (V - V )/ (V - V ) where the the volume at which a component elutes, is the void volume, and is the total volume of the system. Blue dextran 2,000 and xylose were used to determine and Vj respectively. 

Prepararion of the polymeric telluridef One per cent divinylbenzene styrene co-polymer Bio-Beads S-X (Bio-Rad laboratories), is converted into poly(p-lithiostyrene) according to the described procedure. ... 

Transfer anhbodies and antigens from glycerol buffer into glycerol-free PBS solution using spin columns (BioSpin P6, Bio-Rad Laboratories, Hercules, CA). 

Materials NMAAM, (Pfaltz and Bauer), NEAAM, NNBAAM, NTBAAM (Monomer-Polymer and Dajac Laboratories, Inc., Trevose, PA), NIPAAM (Eastman Kodak Company, Rochester, NY), AAM, ammonium persulfate (Bio-Rad Laboratories, Richmond, CA) and TEMED (Sigma Chemical Co., St. Louis, MO) were used as received. NEAAM was also obtained from Polysciences. Sephacryl S-400 was obtained from Sigma. All other chemicals were reagent grade or better. 

Affi-Gel (Bio-Rad Laboratories), Cellthru Big Beads (Sterogene), Mimetic Series (Prometic Biosciences/ACL), Sepharose Big Beads (Amersham/GE Healdicare), Sepharose FF (Amersham/GE Healthcare), Sepharose HP (Amersham/GE Healdicare)... 

Affi-Prep (Bio-Rad Laboratories), Fractogel (Merck), SigmaChrom AF (Supelco), TSK-Gel (TosoHaas/Supelco)... 

Copper negative staining (Copper Stain Bio-Rad Laboratories) Store at 4°C. 

Mouse liver has been widely used for proteome analysis by the 2DE method. (7, 8) In this study, the extracted proteins from the mouse liver tissue are used as an example. Protein spots are evaluated using the gel analysis software ProFINDER 2D (Perk-inElmer Life And Analytical Sciences, Inc., Wellesley, MA) and PDQuest (Bio-Rad Laboratories, Hercules, CA)). 

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