B. abortus

Brucella species including B. abortus B. melitensis B. suis (Agent US)... 

Material Safety Data Sheet-Infectious Substances Brucella spp. (B. abortus, B. canis, B. melitensis,... 

Brucellosis Disease caused by Brucella melitensis, B. mis, B. abortus, or B. canis bacteria species. 

Brucellosis is caused by Brucella melitensis, B. suis, B. abortus, or B. canis bacteria. After entering the human body, the organisms travel from their entrance point to the lymph channels and nodes, eventually reaching the thoracic duct and bloodstream. Carried through the bloodstream, they are deposited eventually in... 

Treatment — A number of antibiotics are available including doxycycline combined with rifampin, and ofloxacin combined with rifampin. Vaccines for animal use directed against B. melitensis and B. abortus have proven very successful. An effective vaccine for human use against B. suis is currently under development.3... 

B. abortus, brucellosis), Flavobacterium, Gluconobacter, Legionella (L. pneumophila, Legionnaire s disease), Methylomonas, Neisseria (N. gonorrhea, gonorrhea), Pseudomonas, Rhizobium, Thermus, Xanthomonas, Rochalimaea (R. henselae, cat scratch disease)... 

In addition to the kinds of activity enumerated above, the highly methoxylated bioflavonoids exhibit a rather strong antibacterial, antifungal, antiviral activity (82-86). The compounds have shown inhibitory activity against organisms such as E. coll, Typhi, Salmonella Typhi, Shigella Dysenteriae, Staphylococcus, B. Abortus Bang, influenza Virus A and rhinoviruses. Some of the compounds were active in concentrations as low as 2.5 pg per liter. 

Figure 4. Preparative 36% and 39% CsCl gradients of Brucella LPS. Shows separation of other components. Left 39% gradient showing B. meletensis LPS. Right 36% gradient showing B. abortus LPS.

Figure 5. 34% CsCl gradients of Brucella LPS. The LPS from B. abortus strain indicated under tube.

Figure 6. Model E analytical schlieren patterns of purified LPS. Equilibrium attained at 36,000 rpm at 25°C for 18 hours in 2°C sector cells. A 34% CsCl pattern of B. abortus LPS, sample from preparation run in Figure 5A. B 34% CsCl pattern of B. abortus LPS. C 39% CsCl pattern of P. multocida LPS.

For the pathogenesis studies, dams were bred with Brucella-negative billies, and their pregnancies later were confirmed via ultrasound examination. Goats in late gestation were exposed conjunctivally to either the virulent parental strains B. melitensis 16M or B. abortus 2308 or the variant B. abortus 2308-QAE with 1 x 107 cfu. Pregnancies were monitored until delivery, and kids were recorded as aborted/weak or live/healthy. 

Dye-sensitivity analysis of B. abortus 2308-QAE and RB51-QAE was typical of that usually seen with B. abortus 2308 andRB51. 

TABLE 1. Colonization of Nonpregnant Goats Inoculated with Brucella abortus 2308, B. abortus 2308-QAE, Brucella melitensis 16M, RB51, or RB51-QAE... 

To assess the pathogenicity of the experimental strains in the ruminant host, pregnant goats in late gestation were exposed to conjunctivally one of the three strains of Brucella. Study results are presented in Table 2. Goats inoculated with B. abortus 2308 displayed a 27% abortion rate as compared with goats infected with B. abortus 2308-QAE, which exhibited a 67% abortion rate (P < 0.05). Additionally, 78% of the animals inoculated with B. melitensis 16M aborted. 

In a recent study, del C Rocha-Gracia and colleagues [5] investigated the ability of various strains of B. abortus and B. melitensis to hemagglutinate human and animal red blood cells. They identified a 29-kd surface protein (SP29) that is associated with the hemagglutination of all of the Brucella strains tested with human (A+ and B+) and animal (rabbit, hamster, rat, and mouse) erythrocytes. 

B. abortus 2308-QAE displayed colonization results typical of vimlent Brucella species in that the parotid lymph node was colonized by 7 days postinoculation. However, B. abortus 2308-QAE infection resulted in a greater number of cfu/g than did its parental B. abortus 2308 strain in animals given the same dosage of infectious organisms -lxlO9 cfu (Table 1). This also held tme for the RB51-QAE strain compared withRB51. 

Serologically, all animals infected with B. abortus 2308-QAE tested positive for the presence of Brucella antibodies in their sera on the brucellosis card test and by Western immunoblot analysis, which used cell lysates from B. abortus 2308, B. abortus 2308-QAE, and B. melitensis 16M. Western blot analysis results were indicative of what is seen typically when the sera from animals exposed to smooth Brucella species are analyzed. RB51 animals all remained negative on the brucellosis card test. 

B. abortus 2308-QAE, the B. abortus 2308 variant, and RB51-QAE also were capable of infecting and colonizing the animal with no sign of attenuation. There was a slight increase in the number of cfu/g of tissues colonized by the organism in comparison with its virulent parental strain 2308 or vaccine strain RB51. 

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