Animal inoculation

Animals inoculated with sterile allantoic fluid should not be housed with Sendai infected animals to prevent cross infection. 

Fig. 3 p -Galactosidase expression in tissues of mice inoculated orally with alginate microspheres containing plasmid DNA (A) liver (B) intestine and (C) spleen sections from the animal inoculated with microspheres containing bovine adenovirus type 3 (BAd3). (D) Liver (E) intestine and (F) spleen sections from the animal inoculated with microspheres containing LacZ plasmid +, BAd3. (From Ref. l)... 

To assess the pathogenicity of the experimental strains in the ruminant host, pregnant goats in late gestation were exposed to conjunctivally one of the three strains of Brucella. Study results are presented in Table 2. Goats inoculated with B. abortus 2308 displayed a 27% abortion rate as compared with goats infected with B. abortus 2308-QAE, which exhibited a 67% abortion rate (P < 0.05). Additionally, 78% of the animals inoculated with B. melitensis 16M aborted. 

Animals inoculated conjunctively usually are colonized in the parotid lymph node within the first 3 days postinfection, with the organism disseminating to the liver and spleen by 7 days postinfection. The supramammary and internal iliac lymph nodes should show signs of colonization by 14 days postinfection [15],... 

Provides a rapid method in which bacteria may be identified specifically within one hour or less after submission of the specimen to the laboratory. This is in contrast to the days or even weeks required for specific identification by conventional techniques, many of which require cultivation to obtain large numbers of organisms in pure culture and even animal inoculation. 

Detection of toxin by mass spectroscopy, animal inoculation, or other methods. 

Although PrP-res is associated with TSE infectivity and has, at the biochemical level at least, some limited self-propagating activity, important questions remain about the PrP-only prion model for the TSE agent. For instance, why has no one been able to demonstrate the model s most basic prediction, that is, that PrP-sen alone can be induced to convert to an infectious agent that causes TSE disease when inoculated into animals Inoculation of a synthetic PrP peptide has induced a TSE-like disease in transgenic mice expressing a familial TSE associated mutant PrP, but the serial transmissibility of this disease remains to be determined (Kaneko et al, 2000). Why are 100,000 PrP-res molecules present per infectious unit Why is PrP-res not always found in infectious tissues (Lasmezas et al, 1997) Why are nucleic acids (Akowitz et al,... 

Tumor Host animal Inoculation Administration 1 Criteria of 2 activity ... 

Diagnosis of Q fever is usually accomplished by serologic testing because culture of C burnetii is potentially hazardous to laboratory personnel and requires animal inoculation or cell culture. 

Bordet, Tchistowitch, Ehrlich, Wassermann and others, and the number now known is very large. If there is injected into an animal a solution of any albumin, serum, milk, egg-albumin, or of a broth of filtered culture, it is found that the serum of the animal inoculated has acquired the property of precipitating the solutions of albumin used to make the injection. These precipitins are specific for example, we have sero-precipitins, lacto-precipitins, anti-globulin precipitins, anti-albumose precipitins, etc. 

The hydro-alcoholic extract of U. tomentosa has shown to be a potential immunostimulant [73]. The extract of this plant was given to BALP/c mice seven times intragastrically with formalin-inactivated whole Sandal virus (S V) [67]. It was noticed that the animals inoculated with 5.6 mg of the dry extract of U. tomentosa induced higher saliva IgA antibodies. In addition, the same amount of extract had significantly higher IgA, IgG, and HI antibody responses to SV than did those administered with the SV alone [74]. The water extracts of U. tomentosa have been shown to induce apoptosis and inhibit proliferation of tumor cells in vitro and to enhance DNA repair in vivo [75,76]. 

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