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Assays primary

Micronucleus assay, primary female Fischer 344 rat hepatocjdes in vitro - NT 74 (3 and 51 h incubation) Reisenbichler Eckl (1993)... [Pg.164]

HTS accelerates the identification of drug leads through the analysis of enormous numbers of samples in a series of automated assays (primary assays) run repetitively by robots. To decide whether to accept or reject the assay results, scientists validate the assay, analyze the data collected, and monitor whether the assay runs according to protocol. Several quality control procedures have been developed to evaluate the screening results and to... [Pg.278]

HIV AIDS Reverse transcriptase and integrase activity assays Primary macrophages, HFFceUs... [Pg.100]

In traditional experiments with the hamster cell clonal assay, primary cultures were prepared from 12- to 14-day-old embryos as needed for each experiment. To enhance plating efficiency, small numbers of cells varying from about 300 to 1000 per dish were seeded onto a sparse lawn of previously X-irradiated feeder cells. Initially, feeder cells were usually from rats, although hamster cells have been used more recently. The cultures were then treated with chemicals for periods of time that varied from a few hours to 8 days, and were then examined for toxicity and transformation. [Pg.182]

The primary use of EIA when it was first developed was for histological labeling and localization of specific cell macromolecules. Eor example, enzymes labeled with peroxidase were used to locate specific cellular compartments and stmctures for microscopic examination. The flexibiUty of EIA was recognized quickly and it was adapted for use as a laboratory assay. [Pg.24]

Biomedical Applications. TRIS AMINO is used for a number of purposes in its pure form, it is an acidimetric standard the USP grade can be utilized intraveneously for therapeutic control of blood acidosis TRIS AMINO also is useful in genetic engineering as a buffering agent for enzyme systems, industrial protein purification, and electrophoresis. AMP has found use as a reagent in enzyme-linked immunoassays. The primary appHcation is for alkaline phosphatase assays. [Pg.19]

Assay Methods. The primary assay for the streptovaricins is the microbiological assay using the agar diffusion method or a turbidimetric procedure (60). The streptovaricins can also be identified by paper (60,88) or thin-layer chromatography (3). [Pg.495]

Standard 1/10 N nitrite is used to titrate a solution prepared by dissolving 10—100 mg of sulfamic acid and about 6 mL of (1 + 1) H2SO4 in 300 mL of distilled water at 40—50°C. At the end point, the colorless external potassium iodide starch-paste indicator changes to blue. A 1-mL solution of 1/ION NaN02 is equivalent to 9.709 mg of sulfamic acid. The 1/10 N nitrite titrant solution is standardized using primary standard-grade sulfamic acid. For sulfamate assay determination, the same procedure is used as for sulfamic acid. [Pg.64]

Bioassays are based on the growth response of vitamin-depleted rats or chicks to graded amounts of vitamin B 2 added in the diet. These assays are not specific for vitamin B 2 because factors, other than vitamin B 2 present in biological materials, produce a growth response. Because coen2yme primary form of natural vitamin 2 is light sensitive, assays should be carried out in subdued light. [Pg.115]

Because the heavy ethyleneamines are very complex materials, assays by titration in aqueous and nonaqueous media are often performed (151). The result is usually expressed as an amine number or amine value, a measure of the total basic nitrogen content of the product. Titrimetric procedures are also available to define primary, secondary, and tertiary amine content (152). [Pg.45]

In vitro cytotoxicity assays using isolated cells have been applied intermittently to cyanobacterial toxicity testing over several years." Cells investigated for suitability in cyanobacterial toxin assays include primary liver cells (hepatocytes) isolated from rodents and fish, established permanent mammalian cell lines, including hepatocytes, fibroblasts and cancerous cells, and erythrocytes. Earlier work suggested that extracts from toxic cyanobacteria disrupted cells of established lines and erythrocytes," but studies with purified microcystins revealed no alterations in structure or ion transport in fibroblasts or erythrocytes,... [Pg.115]

Sensitive to toxins, in this case means that the assay presents no false negative results. Primary hepatocytes can elucidate hepatotoxins, and mouse neuroblastoma cells can elucidate sodium channel-blocking neurotoxins therefore these assays can be used to screen for the appropriate toxins. [Pg.121]

The majority of functional assays involve primary signaling. In the case of GPCRs, this involves activation of G-proteins. However, receptors have other behaviors— some of which can be monitored to detect ligand activity. For example, upon stimulation many receptors are desensitized through phosphorylation and subsequently taken into the cell and either recycled back to the cell surface or digested. This process can be monitored by observing ligand-mediated receptor internalization. For... [Pg.84]

Kunkel, E. J., Plavec, L, Nguyen, D., Melrose, J., Rosier, E. S., Kao, L. T., Wang, Y., Hytopoulos, E., Bishop, A. C., Bateman, R., et al. (2004). Rapid structure-activity and selectivity analysis of kinase inhibitors by Bio Map analysis in complex human primary cell-based models. ASSAY Drug Dev. Technol. 2 431-441. [Pg.197]

Potassium bromate is readily available in a high state of purity the product has an assay value of at least 99.9 per cent. The substance can be dried at 120-150 °C, is anhydrous, and the aqueous solution keeps indefinitely. It can therefore be employed as a primary standard. Its only disadvantage is that one-sixth of the relative molecular mass is a comparatively small quantity. [Pg.406]

Phloretin inhibits FATP-mediated traversing of fatty acids across lipid bilayers. Phloretin is the aglycon of phlorizin and has been used to terminate the uptake of LCFAs and VLCFAs in timed in vitro uptake assays with cultured cells or in ex vivo uptake assays with isolated primary cells. [Pg.498]


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See also in sourсe #XX -- [ Pg.280 ]




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Drug primary activity assays

Hepatocyte primary culture/DNA repair assay

Primary activity assays

Profiling primary assay

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