Analysis of Viruses

For the analysis of viruses recognizing low molecular weight compoimds, one rehes on the application of ligand-based techniques, whereas receptor-... 

VM Okun, R Moser, B Ronacher, E Kenndler, D Blaas. VLDL receptor fragments of different lengths bind to human rhino virus HRV2 with different stoichiometry—an analysis of virus-receptor complexes by capillary electrophoresis. J Biol Chem 276 1057-1062, 2001. 

Because of minimal sample preparation and minimally invasive approaches Raman spectroscopy has the potential to gain new insights into viral and microbial biochemistry as well as a rapid identification of pathogenic microorganisms. In this chapter the unique potential of several Raman spectroscopic techniques for medical and pharmaceutical issues is discussed, which focus on the analysis of viruses as well as on microbial detection... 

Viruses and bacteria are the smallest biologically active identities discovered so far and, naturally, require nanodevices for their profiling. Analysis of viruses and bacteria on microchip devices are of particular interest for experiments with infectious material because of easy containment and disposal of samples. Thus, the use of NCE in routine analyses of viruses and bacteria is exceptionally attractive [171,172], These pathogens are detected based on their different migration times without the need for time consuming sample preparation [173,174]. 

Vims structure and assembly have been topics of intense investigation (Chiu et al, 1997 Steven et al, 1997). The increased activity is partly a result of improved technologies that have facilitated analysis of viruses that were refractory to detailed investigations in the past. Greater computational power and improvements in X-ray radiation sources now permit examination of large and complex viruses by single crystal X-ray analysis... 

C-terminal interactions and, 267-268 X-ray analysis, of virus structure, 1, 3, 221 X-ray crystallography, 93-94, 221, 385-386 cryoelectron microscopy with, 54—56 of southern bean mosaic virus (SBMV), 39 of tomato bushy stunt virus (TBSV), 39 X-ray detectors, 40-41... 

Berkowitz RD, lives H, Plavec I, Veres G. 2001. Gene transfer systems derived from Visna virus analysis of virus production and infectivity. Virology 279 116-29... 

The method used for the rapid analysis of virus isolates relied on the specificity of mAbs. In this study, a relatively large panel was used, the mAbs of which were prepared against several classical type A viruses representing epidemiologically important groups. Thus, one aspect of the study allows an... 

Saltzman RL, Quirk MR, Jordan MC. Disseminated cytomegalovirus infection. Molecular analysis of virus and leukocyte interactions in viremia. J Clin Invest 1988 81(1) 75-81. 

Further reductions can be achieved by taking symmetry into account, an approach that holds promise for the analysis of large oligomeric proteins such as virus capsids [14]. 

Krishnaswamy, S., Rossmann, M.G. Structural refinement and analysis of mengo virus. /. Mol. Biol. 

FIGURE 10.13 Analysis of brome mosaic virus BMV on SynChropak GPC 500. Column 250 X 4.6 mm i.d. Mobile phase 0.05 A1 Bis-Tris, 0.5 A1 sodium acetate, pH 5.9. Flow rate 0.3 ml/min. (Reprinted from Jerson Silva and MICRA Scientific, Inc., with permission.)... 

Phillips, B., Abravaya, K., Morimoto, R.l. (1991), Analysis of the specificity and mechanism of the transcriptional activation of the human hsp70 gene during infection by DNA viruses. J. Virol. 11, 5680-5692. 

Tai CL, Chi WK, Chen DS, Hwang LH (1996) The helicase activity associated with hepatitis C virus nonstructural protein 3 (NS3). J Virol 70 8477-8484 Tong X, Chase R, Skelton A, Chen T, Wright-Minogue J, Malcolm BA (2006) Identification and analysis of fitness of resistance mutations against the HCV protease inhibitor SCH 503034. Antiviral Res 70 28-38... 

Structural analysis of the rhinovirus and the hepatitis A virus 3C proteases (Allaire et al. 1994 Matthews et al. 1994) confirmed earlier predictions that the picomavirus 3C proteases are similar to chymotrypsin-Uke serine proteases in their fold. An important difference is that the serine nucleophile of serine proteases is replaced with a cysteine however, the 3C protease is stracturally distinct from the eukaryotic cysteine protease class of enzymes. 

Analysis of Potential Binding Interactions within the Influenza Virus Sialidase Active Site... 

Smith BJ, Colman PM, von Itzstein M, Danylec B, Varghese JN (2001) Analysis of inhibitor binding in influenza virus neuraminidase. Protein Sci 10 689-696... 

Ohagen A, Devitt A, Kunstman KJ, Gorry PR, Rose PP, Korber B, Taylor J, Levy R, Murphy RL, Wolinsky SM, Gabuzda D (2003) Genetic and functional analysis of full-length human immunodeficiency virus type 1 env genes derived from brain and blood of patients with AIDS. J Virol 77(22) 12336-12345... 

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