Brome mosaic virus

FIGURE 10.13 Analysis of brome mosaic virus BMV on SynChropak GPC 500. Column 250 X 4.6 mm i.d. Mobile phase 0.05 A1 Bis-Tris, 0.5 A1 sodium acetate, pH 5.9. Flow rate 0.3 ml/min. (Reprinted from Jerson Silva and MICRA Scientific, Inc., with permission.)... 

The capped leader sequence of the brome mosaic virus mRNA, an oligonucleotide 5 triphosphate, has been synthesized analogously by reaction of an oligoribonucleotide 5 -phosphoric imidazolide. 951... 

She, Y. M. Haber, S. Siefers, D. L. Loboda, A. Chernushevich, I. Perreault, H. Ens, W. Standing, K. G. Determination of the complete amino acid sequence for the coat protein of brome mosaic virus by time-of-flight mass spectrometry Evidence for mutations associated with change of propagation host. J. Biol. Chem. 2001, 246, 20039-20047. 

Sgro, J., Jacrot, B., and Chroboczek, J. (1986) Identification of regions of brome mosaic virus coat protein chemically cross-linked in situ to viral RNA. Fur. J. Biochem. 154, 69-76. 

The majority of viruses that infect plants have single-stranded, positive-sense RNA genomes. It has therefore been necessary to use infectious cDNA clones for the in vitro manipulation of RNA viruses, allowing them to be developed as effective tools for the commercial production of target proteins in plants. This approach has also been used to study the genetic and metabolic profiles of both viruses and their host plants. Siegel [14] conceptualized the potential use of RNA viruses as expression vectors. Brome mosaic virus (BMV) and Tobacco mosaic vims (TMV) were the first two RNA viruses to be converted into expression vectors. These vectors have since been pro-... 

Brome mosaic virus Icosahedron/28nm diameter [59-61]... 

Brome mosaic viruses (BMV) readily infect different varieties of grasses and to a minor extent maize. Collectively, BMV is among the smallest virases with an icosahedral protein capsid of 27 run in diameter, and in contrast to most other viruses, is cationic at pH below 6.5. The architecture of the capsid portion of the virus is constracted from 180 protein capsomeres that are independently stabilized by weak electrostatic interactions with the negatively charged RNA core (3234, 2865, 2114, and 876 nucleotides). Kao etal. have developed an approach to replacing this RNA core with... 

Figure 14 Dark-field optical micrograph of Au° nanoparticles encapsulted by brome mosaic virus. (Reprinted with permission from Ref. 73. 2003 American Chemical Society)...

As a close relative to the brome mosaic virus the cowpea chlorotic mottle vims (CCMV) possesses an outer diameter of 28 nm, is assembled from 180 subunits that create an icosahedral type structure, and contains an RNA core. " Another similarity is the cationic interior surface of the viral capsid which is formed from subunits with highly basic N-termini (6 positively charged arginine and 3 lysine residues) that project into the cavity and stabilize the RNA core. However as a potential biomineralization template, the electrostatic environment of the virion cavity precludes any cationic metal precursors and thus limits its applicability for nanoparticle synthesis. 

Large-scale fluctuations of an animal virus particle are important for its ability to infect cells, but another type of particle dynamic behavior has been known for decades and was first identified in a plant virus. Brome mosaic virus (BMV), a single-stranded RNA (ssRNA) virus with T=3 icosahedral... 

Dasgupta, R., and Kaesberg, P. (1982). Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and covqiea chlorotic motde virus. Nucleic Acids Res. 10, 703-713. 

Sacher, R., and Ahlquist, P. (1989). Effects of deletions in the N-terminal basic arm of brome mosaic virus coat protein on RNA packaging and systemic infection./. Virol. 63, 4545-4552. 

BMV. See Brome mosaic virus (BMV) BPMV. See Bean pod mottle virus (BPMV) Bragg angle, in cryoelectron microscopy reconstruction, 45... 

Bragg reflection/diffraction point, 40 Brome mosaic virus (BMV), 203 Bromoviridae, 152, 153 BTV. See Bluetongue virus (BTV)... 

FIGURE 1.12 Atomic force microscopy (AFM) also reveals the fundamental periodicity of macro-molecular crystals. In (a) is the surface layer of a crystal of brome mosaic virus, a particle having a diameter of about 280 A. In (b) is an AFM image of a monoclinic crystal of duodecahedral complexes of intact immunoglobulins, which have a diameter of about 230 A. 

Lucas, R. W, Kuznetsov, Y. G., Larson, S. B., and McPherson, A. 2001. Crystallization of brome mosaic virus and t = 1 brome mosaic virus particles following a structural transition. Virology 286,290-303. 

Brome Mosaic Virus (BMV) T = 3 icosahedrai symmetry 180 copies of a singie protein 28 nm in diameter PDB iD 1JS9... 

Figure 4. Autoradiogram of [-methionine labeled peptides synthesized in rabbit reticulocyte lysate and immuno-precipitated by a rabbit anti-insulin serum. The RNAs used were 1 pg of Brome Mosaic Virus as a control (lane 1), no RNA (lane 2), 0.5 yg of polyA+ RNA isolated from rat pancreas (lane 3), 0.2 yg of mRNA synthesized by T7 polymerase (lane 4), 0.2 yg of polyA+ RNA combined with 1 yg of antisense mRNA synthesized by SP6 polymerase (lane 5), and 0.2 yg of synthetic mRNA combined with 1 yg of antisense mRNA. The RNAs used in lane 5 and 6 were heated at 70°C for 15 min and cooled slowly before the translation reaction.

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