Column chromatography, on cellulose

J. R. V ercellolti Extrusion column chromatography on cellulose. J. Chromatogr. [Amsterdam] 18, 42 (1965). 

The helical compounds 198a and 199 were separated into the enantiomers by inclusion chromatography (medium-pressure column chromatography on cellulose triacetate). 200 was enantiomerically enriched (—)-200 was only weakly enrich-... 

The disadvantage of using the nondialyzable fraction as the starting material for further fractionation is evident from the work of Boas,8 who found that 47 % of the total, urinary aminodeoxyhexose is dialyz-able in preliminary studies, he resolved three glycopeptide-contain-ing fractions in the dialyzable, urinary material by means of column chromatography on cellulose he commented on the difficulty encountered in fractionating this material, since the aminodeoxyhexose accounts for only 0.002% (w/w) of the dialyzable, urinary solids. 

The total lipids thus obtained usually still contain free amino acids and peptides, sugars and other hydrophilic, naturally occurring material which are carried into the extract through the action of solubilisers like lecithin. Column chromatography on cellulose or Sephadex is suitable for removal of these contaminants. 

Column chromatography on cellulose - or dextran -based ion exchangers offers new possibilities of efficient pre-fractionation. 

Dipeptidyl aminopeptidase (from rat brain) [9031-94-1] [EC 3.4.11.10]. Purified about 2000-fold by column chromatography on CM-cellulose, hydroxylapatite and Gly-Pro AH-Sepharose. [Imai et al. J Biochem (Tokyo)93 431 1983.]... 

Purification of aequorin. The purification method of aequorin reported by Shimomura et al. (1962) was essentially the repetition of column chromatography on DEAE-cellulose, the only usable, efficient chromatographic adsorbent available at the time. Since then, various different types of chromatographic media have been developed, and the purification method has been steadily improved. 

Fig. 4a. Resolution of racemic (67) by column chromatography on microcrystalline cellulose triacetate (column B) and by recrystallization 35) C = crystals M = mother liquor the index following C or M gives the number of recrystallizations the fraction has undergone. The value of [ot] ° is given, followed by the quantity obtained...

Finally, it should be noted that several chiral tetraorganostannanes have been partially resolved by column chromatography on microcrystalline cellulose triacetate22. 

Salicylic acid and Its metabolite were separated by two methods. The first was thin layer chromatography on cellulose with BAW solvent as for the In vivo metabolism studies. A quicker separation was achieved with a polyamide column. The entire 400 pL from an individual assay was placed on top of a 0.8 x 2.0 cm column packed with Polyamide-6 (Accurate Chemical and Scientific Corp.). The salicylic acid metabolite was eluted with 6 mL water but salicylic acid was retained. 3a70B scintillation fluid (Research Product International Corp.) was used to determine the radioactive content of the entire 6 mL of eluant. Separation of salicylic acid and its metabolite by polyamide column chromatography was verified by thin layer chromatography. 

Schober, R. and Heimburger, N. i960. Fractionation of Na-caseinate by column chromatography on ion-exchange-cellulose. Milchwissenschaft 15, 607-609 (German). 

Kaul and Lester (10) reported the preparation of six novel glycophosphoceramide fractions from the above crude concentrate from tobacco leaves. The crude concentrate was first resolved into two groups by column chromatography on diethyl aminoethyl-cellulose. The first group contained no acetyl residues, whereas the second group contained one N-acetyl per phosphorus. Three lipid fractions from each group were further resolved by chromatography on Porasil columns. The chemical composition and the percent of the total P in the crude concentrate of these lipid frac-... 

Mixtures of methylated sugars are generally fractionated by partition chromatography on cellulose columns. Lemieux and coworkers have found that columns of diatomaceous earth (for example, Celite) are as good as, or better than, cellulose columns for these separations. 

Fig. 15. Horizontal starch gel electrophoresis to show the small differences in mobility between three fetal hemoglobin variants. Left to right (1) Hb-F-Malta-I heterozygote, (2) and (3) normal adults (4) Hb-Fx in a Negro newborn (5) Hb-F-Malta-II heterozygote (6), (7), and (8), Hb-A, Hb-F, and Hb-F-Malta-II fractions, respectively, isolated by column chromatography on CM-cellulose. Tris-EDTA-boric acid buffer, pH 9.0, o-dianisidine stain.

Solutions of oligonucleotides, particularly small ones, can be concentrated by column chromatography on DEAE-cellulose or charcoal. DEAE-cellulose is much more widely used. The concentrated solution contains 1 or 2 M ammonium bicarbonate or other... 

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