Cross-linked dextran

Dextran cross-linked functionalized by carboxymethylation 10-30 Intratumor direct delivery Doxorubicin mitomycin... 

Gels used for the stationary phase can be hydrophilic, for separations in aqueous and other polar solvents, or hydrophobic, for use in non-polar or weakly-polar solvents. Agar, starch, polyacrylamide and cross-linked dextrans possess hydroxyl or amide groups and are thus hydrophilic. They swell in aqueous media and in such solvents as ethylene glycol and dimethylform-amide. Bio-Cel (a co-polymer of acrylamide and jV.W -methylene-bisacryl-amide) and Sephadex (dextran cross-linked with epichlorhydrin) arc two commercially available gels made in bead form. Cross-linking produces a... 

One of the most popular gel filtration materials is Sephadex, supplied by Pharmacia, which consists of dextran cross-linked by epichlorhydrin this is supplied in dried form which must be pre-swollen in water or buffer before use. The quantity of water taken up by the Sephadex partides depends on the degree of cross-linking, as does the time taken to complete the process swelling is more rapid at higher temperatures. The porosity of the material determines the exdusion limit G-10, G-15,... 

Sephacryl HR is an allyl-dextran cross linked with bis-acrylamide. The gd partides have a diameter ranging from 25-75 pm and are mechanically very rigid. 

The trade names of the stationary phase media represent particular polymers formed under well-controlled conditions. Sephadex consists of dextran, cross-linked with epichlorohydrin in alkaline solution. Sephacryl is an allyl dextran, cross-linked with iV.iV -methylenebislacrylamide). Sepharose is a beaded form of agarose gel, where aggregates of the polysaccharide chains are formed. 

Sephadex ion exchangers are suitable for separation of large biological molecules. These are prepared from the polysaccharide dextran cross-linked by reaction with epichlorohydrin, followed by the introduction of the desired functional groups on the fiber and pore surfaces [21]. 

Sephacryl S-100 HR Pharmacia Dextran cross-linked with bisacrylamide 2-11 1-120 25-75 1-100 15... 

Sephadex G-10 Dextran cross-linked with epichlorohydrin 2-10 1-120 <0.7 5... 

Cortesi, R., Esposito, E., Osti, M., Squarzoni, G., Menegatti, E., Davis, S. S., Nastruzzi, C. (1999). Dextran cross-linked gelatin microspheres as a drug delivery system, Eur. I. Pham. Bionharm.. 47,153-160. 

Dextran cross-linked with epichlorohydrin Sephadex G Aqueous 0.1 A/ or 0.5 M NaOH Kraft Ugnin [1,4]... 

Ion filtration chromatography is a time-saving combination of ion exchange and gel chromatography without a salt gradient. P. are separated on diethyl-aminoethyl-(DEAE)-dextran (cross-linked) at a constant pH within 2 to 3 hours. 

Sephadex (a dextran cross-linked with epichlorohydrin) has been used as an affinity matrix for the separation of [ I]-concanavalin A from the unlabelled lectin. If this technique is successful with other I-labelled proteins, it would help to overcome the uncertainty of whether or not biological properties displayed by labelled materials arise from contamination by the unlabelled material. 

Acetylated forms of Sephadex (dextran cross-linked with epichlorohydrin) have been used in the isolation of various proteolipids, and Sephadex has been etherified by reaction with alkaline sodium borohydride and 1-allyloxy-2,3-epoxypropane. Treatment of the etherified Sephadex with mercuric acetate gave a polymer containing covalently-bound, monofunctional mercury, which was used in the separation of nucleotides. 

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