Yersinia pestis detection

Thulasiraman, V., McCutchen-Maloney, S.L., Motin, V.L., and Garcia, E., Detection and identification of virulence factors in Yersinia pestis using SELDl Frotein-Chip system, Biotechiques, 30, 428 32, 2001. 

J.M. Libby and H.G. Wada, Detection of Neisseria meningitidis and Yersinia pestis with a novel sihcon-based sensor, J. Clin. Microbiol., 27(7)(1989) 1456-1459. 

In one report, an array of antigens patterned on a substrate was constructed for detection of IgG antibodies from various species. This method, termed miniaturized mosaic immunoassay, is rendered possible by using the PDMS channel for patterning materials [1027]. In another report, various antibodies were patterned via the PDMS channel on a substrate for detection of three antigens FI antigen (from Yersinia pestis), staphylococcal enterotoxin B, and D-dimer (DDi, a marker of sepsis and thrombotic disorder) [1028],... 

For detection of especially dangerous bacterial and viral agents such as Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella species and influenza A/H5, real-time PCR using a LightCycler 2.0 (Roche) and reagents (Idaho Technologies) has been applied at NCDC. 

Drancourt, M., Aboudharam, G., Signoli, M., Dutour, O., Raoult, D. 1998. Detection of 400-year-old Yersinia pestis DNA in human dental pulp an approach to the diagnosis of ancient septicemia. Proc. Natl. Acad. Sci. USA 95 12637-12640. 

A mouse monoclonal antibody directed against the fraction 1 antigen of Yersinia pestis has been used to detect intracellular and extracellular bacteria in dermal blood vessels, lungs, lymph nodes, spleen, and liver (Fig. 

Guarner J, Shieh WJ, Greer PW, et al. Immunohistochemical detection of Yersinia pestis in formalin-fixed paraffin-embedded tissue. Am J Clin Pathol. 2002 117 205-209. 

Fig. 9.5.7 Direct detection and amplification of the bacterium Yersinia pestis (non-pathogenic strain, irradiated) at 1 x 10 colony forming units (CFU) per mt. The upper line is an average of three sensors with an anti-Vf pestis antibody surface. The lower line is an average of three channels with a reference antibody on the surface. Arrows indicate when sample is injected and when the amplifier was injected into the 24-channel box.

Lindler L. E., Fan W., and Jahan N., Detection of ciprofloxacin-resistant Yersinia pestis by fluorogenic PCR using the light cycler, J. Clin. Microbiol., 39(10), 3649-3655,... 

HMMWV) and equipped with a biological detection suite (Figure 16-32). In its present configuration, the BIDS can detect the bacteria Bacillus anthracis and Yersinia pestis, and the toxins botuli-num toxin A and staphylococcal enterotoxin B. 

Hinnebusch J, Schwan TG. New method for plague surveillance using polymerase chain reaction to detect Yersinia pestis in fleas. J Clin Microbiol. 1993 31 (6) 1511 — 1514. 

Higgens JA, Ezzell J, Hinnebusch BJ, Shipley M, Henchal EA, Ibrahim MS. 1998. 5 nuclease PCR assay to detect Yersinia pestis. Journal of Clinical Microbiology 36 2284-2288. 

Meyer M.H.F., Stehr M., BhujuS., Krause H.J., Hartmann M., Miethe R, Singh M., and Keusgen M. 2007b. Magnetic biosensor for the detection of Yersinia pestis. Journal of Microbiological Methods 68 218-224. 

Chenau J, Fenaille F, Simon S, Filali S, Volland H, Junot C, Camiel E, Becher F. Detection of Yersinia pestis in environmental and food samples by intact cell immunocapture and liquid chromatography-tandem mass spectrometry. Anal Chem. 2014 86(12) 6144-52. doi 10.1021/ ac501371r. 

J M Libby and G W Wada, Detection of Neisseria Meningitidis and Yersinia Pestis with a Novel Silicon Based Sensor, J Clin Micro, 27 (1989) 1456-1459... 

Wojciechowski J, Danley D, Cooptr J, Yazvenko N, Rowe Taitt C (2010) Multiplexed electrochemical detection of yersinia pestis and staphylococcal enterotoxin B using an antibody microarray. Sensors 10 3351-3362... 

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