Virus capsids

Conway J F, Cheng N, Ziotnick A, Wingfieid P T, Stahi S J and Steven A C 1997 Visuaiization of a 4-heiix bundie in the hepatitis B virus capsid by cryo-eiectron microscopy Nature 386 91-4... 

Further reductions can be achieved by taking symmetry into account, an approach that holds promise for the analysis of large oligomeric proteins such as virus capsids [14]. 

Rayment, I., et al. Polyoma virus capsid structure at 22.5 A resolution. Nature 29S 110-115, 1982. 

X-ray crystallographic structure of the Norwalk virus capsid. Science 286, 287-290. 

Immunocytochemical methods were developed to detect specific antibodies in sera from patients affected by different infectious diseases. P3-HR1 cells, which express Epstein-Barr-virus-induced virus capsid antigens (VCA), were used to search for specific human IgM (class M immunoglobulins) to VCA in infectious mononucleosis patients. After treatment of cells with serial dilutions of sera, HRP-conjugated anti-IgM antibody was added and detected with CL substrate [25],... 

UV VC A VE YFP Ultraviolet Virus capsid antigens Vitamin E Yellow fluorescent protein... 

M. A. Tito, K. Tars, K. Valegard, J. Hajdu, and C. V. Robinson. Electrospray Time-of-Flight Mass Spectrometry of the Intact MS2 Virus Capsid. J. Am. Chem. Soc., 122(2000) 3550-3551. 

Tito, M. A., Tars, K., Valegard, K., Hadju, J., Robinson, C. V Electrospray time-of-flight mass spectrometry of the intact MS2 virus capsid. J Am Chem Soc 2000, 122, 3550-3551. 

Conway, J. R, et al. (1997). VisuaUzation of a 4-hehx bundle in the hepatitis B virus capsid by cryo-electron microscopy. Nature 386,91-94. 

NT070 Mason, H. S., J. M. Ball, J. J. Shi, X. Jiang, M. K. Estes, and C. J. Amtzen. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA 1996 93(11) 5335-5340. 

Martin-Alonso, J.M., Castanon, S., Alonso, R, Parra, E, and Ordas, R. (2003). Oral immunization nsing tuber extracts from transgenic potato plants expressing rabbit hemorrhagic disease virus capsid protein. Transgenic Res. 12 127-130. 

Zhang, X., Buehner, N.A., Hutson, A.M., Estes, M.K., and Mason, H.S. (2006). Tomato is a highly effective vehicle for expression and oral immunization with Norwalk virus capsid protein. Plant Biotechnol J. 4(4) 419 32. 

Ried, M.U., A. Girod, K. Leike, H. Buning, and M. Hallek, Adeno-associated virus capsids displaying immunoglobulin-binding domains permit antibody-mediated vector retargeting to specific cell surface receptors. J Virol, 2002. 76(9) 4559-66. 

In collaboration with the laboratory of virology of the Institute of Molecular and Cellular Biology of Strasbourg, our laboratory studied the ultrasonic absorption of the BMV (Brom mosaic virus) capside and of the dissociated system. The capside is made of 180 proteins it can easily be dissociated into 90 dimers. 

A certain class of virus capsides (protein shells of virions) have a spherical structure, that is modeled on dual GCk,i(Dodecahedron) (see [CaK162, Cox71, DDG98]). 

Oscillation photography can be used in tandem with area detectors, but detector resolution limits the size of the unit cell from which data can be collected. Large unit cells, such as those of virus capsids, mean small reciprocal unit cells and large numbers of closely spaced reflections. Image plates are commonly used in such cases because of their greater spatial resolution. 

Dohner K, Wolfstein A, Prank U, Echeverri C, Dujardin D, Vallee R, Sodeik B (2002) Function of dynein and dynactin in herpes simplex virus capsid transport. Mol Biol Cell... 

Electrochemical studies confirmed the presence of redox-active nanoparticles. Differential pulse and cyclic voltammetry studies were conducted. Cyclic voltammetry showed that the complex displays an electrochemically reversible ferrocene/ ferrocenium couple (Figure 9.6). The oxidation potential for the hybrid CPMV-Fc conjugate and free ferrocenecarboxylic acid in solution was determined E1/2 of CPMV-Fc was 0.23 V, and Elj2 of free ferrocenecarboxylic acid was 0.32 V versus the Ag/AgCl electrode, respectively. This shift is expected for the conversion of the carboxyl group of ferrocenecarboxylic acid to an amide on coupling to the virus capsid, since the amide is less electron-withdrawing. 

PE has molecule-specific links to external resources, for example to visualize specific oligomers or virus capsids, or to color by evolutionary conservation or mutation. 

Wang MY, Kuo YY, Lee MS, Doong SR, Ho JY, Lee LH (2000), Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography, Biotechnol. Bioeng. 67 104-111. 

White LJ, Hardy ME, Estes MK (1997), Biochemical characterization of a smaller form of recombinant Norwalk virus capsids assembled in insect cells, J. Virol. 71 8066-8072. 

Kegel, W.K., and van der Schoot, P. "Competing hydrophobic and screened Coulomb interactions in hepatitis b virus capsid assembly". Biophys.. 86, 3905-3913 (2004). 

---