Vector Escherichia coli

Fig. 1 Schematic outline of procedures employed in the synthesis of a cDNA gene copy from a polyadenylated mRNA template, insertion of the cDNA into a bacterial plasmid vector by a homopolymer tailing strategy, and cloning of the recombinant plasmid in an Escherichia coli host.

Denis-Larose, C. Bergeron, H. Labbe, D., et al., Characterization of the Basic Replicon of Rhodococcus Plasmid pSOX and Development of a Rhodococcus Escherichia Coli Shuttle Vector. Applied and Environmental Microbiology, 1998. 64(11) pp. 4363-4367. 

Mergulhao, F.J.M., Monteiro, G.A., Cabral, J.M.S., and Taipa, M.A. 2004. Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli. Journal of Microbiology and Biotechnology. 14, 1-14. 

Expression cloning, 10 263, 10 264 Expression profiling, 13 354-355 Expression vectors, 12 474 in Escherichia coli, 12 476 Ex situ bioremediation defined, 3 759t... 

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

Amann, E., Brosius, J., and Ptashne, M. (1983). Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25, 167-178. 

Staub, J.M. and Maliga, R. (1995). Marker rescue from the Nicotiana tabacum plastid genome using a plastid Escherichia coli shuttle vector. Mol. Gen. Genet. 249 37 2. 

Farinha, M.A. Kropinski, A. M. (1989). Construction of broad-host-range vectors for general cloning and promoter selection in Pseudomonas and Escherichia coli. Gene, 11, 205-10. 

Skerra, A., Pfitzinger, I, and Pluckthun, A. (1991) The functional expression of antibody Fv fragments in Escherichia coli-improved vectors and a generally applicable purification technique. Biotechnology 9, 273—278... 

Nakamura, K., Iwasaki, Y. Hattori, T. (1980). An improved Escherichia coli expression vector for the construction and identification of full-length cDNA clones. Gene 44, 347-51. 

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