Escherichia coli expression vector strains

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

Ryan, E.T. Crean, T.I. John, M. Butterton, J.R. Clements, J.D. Calderwood, S.B. Vivo expression and immunoadjuvancy of a mutant of heat-labile enterotoxin of escherichia coli in vaccine and vector strains of vibrio cholerae. Infect. Immun. 1999, 67, 1694—1701. 

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