Vectors broad-host-range

Construction of a broad host range cloning vector and its use in the genetic anlysis of Rhizobium mutants. Gene 18 289-296... 

The sindbis virus is simple, robust, capable of infecting non-dividing cells and generally supports high levels of gene expression. However, it does display a broad host range and, hence, lacks the inherent targeting specificity characteristic of an idealized viral vector. 

Isolation and sequencing of the cellulose synthase gene(s) has not been accomplished yet however, DNA from Acetobacter xylinum containing this gene(s) was cloned into broad host-range plasmid vectors (82). These vectors were mobilized into Pel- mutants to test for complementation. To date, this approach has not produced a pellicle-forming transconjugant from a Pel- mutant of Acetobacter (82). The direct correlation between cellulose production and presence of plasmid DNA in Acetobacter has been reported... 

Farinha, M.A. Kropinski, A. M. (1989). Construction of broad-host-range vectors for general cloning and promoter selection in Pseudomonas and Escherichia coli. Gene, 11, 205-10. 

Schweizer, H. P. (1991). Improved broad-host-range /ac-based vectors for the isolation and characterization of protein fusions in Pseudomonas aeruginosa. Gene, 103, 87-92. 

R Sukchawalit, P Vattanaviboon, R Sallabhan, S Mongkolsuk. Construction and characterization of regulated L-arabinose-inducible broad host range expression vectors in Xanthomonas. FEMS Microbiol Lett 181 217-223, 1999. 

Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, Peterson KM. Four new derivatives of the broad-host-range cloning vector pBBRlMCS, carrying different antibiotic-resistance cassettes. Gene 1994 166 800-2. 

E, and Jaeger, K.E. (2012) Novel broad host range shuttle vectors for expression in Escherichia coli. Bacillus subtilis and Pseudomonas putida. J. Biotechnol, 161 (2), 71-79,... 

Plasmids of the incompatibility groups C, N, P, Q and W exhibit a host range much widm than those commonly used as iq>licons for E. co/l-specific vectors. Their properties have been reviewed in several recent articles that provide lists of vectors and hosts (34,48,49). Vectors based on IncP and IncQ replicons are among the most highly developed broad-host-range vectors available to date. For some of them, such as pMMB66 and pMMB207 smes the entire nucleotide sequence has been determined (34). 

The genes for both of the lactate dehydrogenase enzymes, l-LDH and d-LDH, that control lactic acid stereospecificity in the lactobacilli have been cloned and sequenced. The l-LDH enzyme from a variety of lactic acid bacteria forms a closely related class entirely distinct from the d-LDH enzymes which form a second closely related group of proteins. With broad host-range integration vectors now available, it is possible to... 

Labes M, Puhler A, Simon R (1990) A new family of RSFlOlO-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria. Gene 89(l) 37-46 Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Bryant SH (2011) CDD a conserved domain database for the functional annotation of proteins. Nucleic Acids Res 39(Database issue) D225-229... 

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