Escherichia coli expression vector

Nakamura, K., Iwasaki, Y. Hattori, T. (1980). An improved Escherichia coli expression vector for the construction and identification of full-length cDNA clones. Gene 44, 347-51. 

Skerra, A., Pfitzinger, I, and Pluckthun, A. (1991) The functional expression of antibody Fv fragments in Escherichia coli-improved vectors and a generally applicable purification technique. Biotechnology 9, 273—278... 

Walsh, M.K. and Swaisgood, H.E. 1994. An Escherichia coli plasmid vector system for production of streptavidin fusion proteins expression and bioselective adsorption of streptavidin-(3-galactosidase. Biotechnol. Bioengng 44, 1348-1354. 

W, and Sahm, H. (1991) A family of Corynehacterium glutam-icum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing. Gene, 102 (1), 93-98. 

Expression cloning, 10 263, 10 264 Expression profiling, 13 354-355 Expression vectors, 12 474 in Escherichia coli, 12 476 Ex situ bioremediation defined, 3 759t... 

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

Amann, E., Brosius, J., and Ptashne, M. (1983). Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25, 167-178. 

M Inouye, Y Takada, N Muto, T Beppu, S Horinouchi. Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector. Mol Gen Genet 245 456-464, 1994. 

Escherichia coli transformed by such expression vectors... 

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