Lambda vectors

Fig. 1. Differential screening of a human cDNA library with subtracted PolyA cDNA probes. A human cDNA library is prepared using the lambda cloning vector Lambda ZAP (Stratagene). Two replica filters are produced from a single petri dish containing approximately 2,000 recombinant clones. One filter is hybridized with a probe enriched for sequences expressed in unilineage cells (U-M), and the other hybridized with a probe enriched for sequences expressed in multilineage cells (U-M). The arrows indicate clones that were hybridized strongly with one probe and not the other.

Fig. 4. Construction of recombinant phage in vectors derived from bacteriophage lambda where E represents the enzyme EcoRl. Other terms are defined...

Christensen, A. 2001. Bacteriophage lambda-based expression vectors. Molecular Biotechnology 17, 219-224. 

Lambda-cyhalothrin, in microcapsule formulations, 7 564t Lambda-derived cloning vectors, 12 504-506 Lambda sensor, 10 56 Lambent, commercial defoamer, 3 24 It Lambert-Beer-Bouguer law, 23 126. See also Beer s Law... 

In one mutated form of lambda the repressor protein is inactive at temperatures above 37 °C but active at lower temperatures. When this lambda is used as a vector the infected E. coli are grown first at 32 °C to allow replication of the DNA in the lysogenic cycle. The temperature is then increased to 37 °C to inactivate the repressor. This results in the excision of the lambda genome and release of lambda particles by lysis. 

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. 

E. coli host strain AR58 containing defective phage lambda lysogen transformed with a recombinant vector which carries antibiotic resistance for kanamycin (Kan R). 

Rot(axis,theta,c) rotates vector c around axis by theta c=Rot(axis,theta,c-a)/sqrt(lambda)+[0.5,0.5,0.5] Outputlmage[x,y]=c... 

Jaspers LS, De Keyser A, Stanssens PE, Lambda ZLG6 A phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage, Gene, 173 179-181, 1996. 

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