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Lambda vectors

Fig. 1. Differential screening of a human cDNA library with subtracted PolyA cDNA probes. A human cDNA library is prepared using the lambda cloning vector Lambda ZAP (Stratagene). Two replica filters are produced from a single petri dish containing approximately 2,000 recombinant clones. One filter is hybridized with a probe enriched for sequences expressed in unilineage cells (U-M), and the other hybridized with a probe enriched for sequences expressed in multilineage cells (U-M). The arrows indicate clones that were hybridized strongly with one probe and not the other. Fig. 1. Differential screening of a human cDNA library with subtracted PolyA cDNA probes. A human cDNA library is prepared using the lambda cloning vector Lambda ZAP (Stratagene). Two replica filters are produced from a single petri dish containing approximately 2,000 recombinant clones. One filter is hybridized with a probe enriched for sequences expressed in unilineage cells (U-M), and the other hybridized with a probe enriched for sequences expressed in multilineage cells (U-M). The arrows indicate clones that were hybridized strongly with one probe and not the other.
Fig. 4. Construction of recombinant phage in vectors derived from bacteriophage lambda where E represents the enzyme EcoRl. Other terms are defined... Fig. 4. Construction of recombinant phage in vectors derived from bacteriophage lambda where E represents the enzyme EcoRl. Other terms are defined...
Christensen, A. 2001. Bacteriophage lambda-based expression vectors. Molecular Biotechnology 17, 219-224. [Pg.54]

Lambda-cyhalothrin, in microcapsule formulations, 7 564t Lambda-derived cloning vectors, 12 504-506 Lambda sensor, 10 56 Lambent, commercial defoamer, 3 24 It Lambert-Beer-Bouguer law, 23 126. See also Beer s Law... [Pg.508]

In one mutated form of lambda the repressor protein is inactive at temperatures above 37 °C but active at lower temperatures. When this lambda is used as a vector the infected E. coli are grown first at 32 °C to allow replication of the DNA in the lysogenic cycle. The temperature is then increased to 37 °C to inactivate the repressor. This results in the excision of the lambda genome and release of lambda particles by lysis. [Pg.466]

Armed with this new tool, Schena et al. (1996) created a microarray of 1,046 human cDNAs of unknown sequence. They were derived from human peripheral blood lymphocyfes fransformed wifh Epsfein-Barr virus. Suitably sized inserts [>600 base pairs (bp)] were cloned into a lambda vector, subsequently infected into an Escherichia coli strain, and finally amplified by polymerase chain reaction (PCR) using 5 -amino-modified primers. The resulting 5 -amino-modified cDNA amplicons were then arrayed onto sily-lated microscope slides. Next, the expression levels in human Jurkat cells undergoing heat shock or phorbol ester induction were examined. [Pg.148]

E. coli host strain AR58 containing defective phage lambda lysogen transformed with a recombinant vector which carries antibiotic resistance for kanamycin (Kan R). [Pg.6]

Rot(axis,theta,c) rotates vector c around axis by theta c=Rot(axis,theta,c-a)/sqrt(lambda)+[0.5,0.5,0.5] Outputlmage[x,y]=c... [Pg.354]

Jaspers LS, De Keyser A, Stanssens PE, Lambda ZLG6 A phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage, Gene, 173 179-181, 1996. [Pg.406]


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See also in sourсe #XX -- [ Pg.1496 ]

See also in sourсe #XX -- [ Pg.43 ]




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