Tryptone Soya Broth medium

Inoculate a sample of the bacterium from an agar slope into a medium of 9 mL Tryptone Soya Broth or Nutrient Broth and incubate overnight in an orbital shaker at 37°C. 

NOTE The type of broth used is often sterile tryptone soya broth that may be presented in double strength to allow for dilution with buffer, saline, or water to simulate the process. Any suitable liquid culture medium may however be used but the ability of the broth to support growth should be demonstrated. 

Media are prepared to defined formulations using requisite amounts of appropriate chemicals. Many of the more commonly used media, such as nutrient broth, tryptone soya broth, malt agar, and potato dextrose agar, are available in pre-mixed dehydrated form from various specialist suppliers. Any components of media that are heat-sensitive must be added to the remainder of the medium after it has been heat-sterilized by autoclaving this is normally done by syringing in these particular components as concentrated aqueous solutions through disposable sterile filter units available from various specialist suppliers. 

Both organisms were grown in TSB medium, consisting of Tryptone Soya Broth (Oxoid Ltd. 3%, w/v) supplemented with inorganic ions and vitamins as described by Abbas-Ali and Coleman (1977). Batches of medium (50ml), contained in 250 ml conical flasks, were inoculated with bacteria from Tryptone Soya Agar (Oxoid Ltd.) slopes by means of a platinum loop. The cultures were incubated in a "Gyratory" incubator-shaker (model G25, New Brunswick Scientific Co., New Brunswick, N.J., U.S.A.) at 37 C in the case of S. aureus and 25 C for A. salmonicida. 

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