Broth tryptone

Luria-Bertani (LB) broth tryptone (35 g) yeast extract (17.5 g)... 

Media are prepared to defined formulations using requisite amounts of appropriate chemicals. Many of the more commonly used media, such as nutrient broth, tryptone soya broth, malt agar, and potato dextrose agar, are available in pre-mixed dehydrated form from various specialist suppliers. Any components of media that are heat-sensitive must be added to the remainder of the medium after it has been heat-sterilized by autoclaving this is normally done by syringing in these particular components as concentrated aqueous solutions through disposable sterile filter units available from various specialist suppliers. 

LB broth 10 g/L Bacto-tryptone, 5 g/L Bacto-yeast extract, 10 g/L NaCl, adjusted to pH 7.0 with NaOH and sterilized by autoclaving. 

Yeast-tryptone (YT) broth (1% Bactotryptone, 0.5% yeast extract, 0.5% NaCl)... 

Luria broth—Luria broth Prepare five 100-ml and five 10-liter solutions containing 10 g/liter Bacto-Tryptone, 5 g/liter Bacto-Yeast extract, 5 g/liter NaCl, 0.5 g/liter antifoam agent (e.g., Polyglycol P-2000), and 1 g/liter glucose (autoclave separately). Autoclave the main solution and separate solution of concentrated glucose for 20 min at 105 to 110°C and cool use sterile conditions to add the glucose solution to the main solution and use within 24 hr. 

L-Broth (1L) 10gofbacto-tryptone,5gofyeastextract,5gofNaCl,and 2.4 g of MgS04.7H20. Adjust to pH 7.5. 

Erwinia herbicola NCIMB 12126 was obtained from the National Collection of Industrial and Marine Bacteria (Aberdeen, UK), HEPES buffer sachets and magnesium acetate were obtained from Sigma (Poole, UK), adenylate kinase assay kits were obtained from Acolyte Biomedica (Salisbury, UK), sterile tissue culture grade distilled water was obtained from Gibco (Paisley, UK), L-broth and tryptone soya agar plates were obtained from Oxoid (Basingstoke, UK). 

Escherichia coli NCIMB 10243 was grown overnight in Tryptone Soya Broth (Oxoid CM129) in an orbital shaker at 35 C. This was used to inoculate water samples containing biocides (2 mL of culture added to 18 mL sample). Final concentrations of the biocides were 200 ppm. Samples were taken from the biocide/bacteria solutions at various time-points and both ATP assays and plate counts performed. 

The plate counts at To were estimated by diluting the neat broth culture 1 in 10 with Neutralised Peptone Water (NPW) containing per L 1.0 g Bacteriological Peptone (Oxoid, L37), 8.8 g Sodium Chloride (May Baker), 3.0 g Amisol 910 (Degussa) and 30.0 g Tween 80 (BDH, 560234H) and then decimally with Phosphate Buffered Saline (Oxoid). For biocide treated samples 1 mL was diluted in 9 mL of NPW, mixed and allowed to stand for 5 min (for neutralisation of the biocide). The solution in NPW was diluted decimally (0.1 mL in 0.9 mL) in PBS. Appropriate dilutions (0.1 mL) were plated out on Tryptone Soya Agar Plates (bioMerieux) and incubated at 37 °C for 24 h. 

Inoculate a sample of the bacterium from an agar slope into a medium of 9 mL Tryptone Soya Broth or Nutrient Broth and incubate overnight in an orbital shaker at 37°C. 

NOTE The type of broth used is often sterile tryptone soya broth that may be presented in double strength to allow for dilution with buffer, saline, or water to simulate the process. Any suitable liquid culture medium may however be used but the ability of the broth to support growth should be demonstrated. 

Grow E. coli cells at 32°C for best motility. Higher temperatures reduce motility. The media used for growth is important. Using the wrong media can poison or reduce the motility of the bacteria. All our experiments used tryptone broth. 

LB Broth 10 g NaCl, 10 g tryptone, 5 g yeast extract in 1 1 of pH 7.0 adjusted with NaOH. Autoclave and store at room temperature. Stable for 1 year. 

Tryptone soy broth (TSB) (Oxoid, Wesel, Germany), autoclaved for 15 min. 

For the host-mediated test, tryptone broth was inoculated from an agar slant culture of S. typhimurium and incubated in a reciprocating shaker for 2 hr at 37°C. This culture was diluted 1 4 with saline (final ODeeo approximately 0.1), and 2 ml of the resulting suspension was injected intra-peritoneally into the mouse. 

Inoculate 50 ml of sterile L Broth (5 g yeast extract, 8 g bacto-tryptone, 5 g NaCl to 11 with H2O) with single colony of desired bacterial strain. 

Potato dextrose agar Tween-80 Tryptone WL-nutrient agar WL-differential agar Wort agar Yeast carbon base Yeast extract YM agar YM broth... 

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