Trichoderma reesei production

Production of Trichoderma reesei Cellulase System with High Hydrofytic Potential by Solid-State Fermentation... 

Table III. Cellulase Production on Aspen Pulp (CTMP) with Trichoderma reesei QMY-1...

It has been proposed that the production of xylanases and cellulases is under separate regulatory control in some filamentous fungi (1). Hrmova et al. (42) reached a similar conclusion after monitoring the daily production of these enzymes in Trichoderma reesei QM 9414. Xylanase and cellulase activities followed independent production profiles during fungal growth. The same effect has been observed in batch cultures of T. harzianum. We have observed peak xylanase activity on the third day of growth whereas the cellulase activity peaked after day five or six (unpublished). 

Potato pulp and potato pulp residue (after acidic treatment) and nutrient salt solution and potato protein liquor, respectively, were used for the production of cellulases and hemicellulases by Trichoderma reesei Rut C30 in a continuously operated bioreactor, (Klingspohn and Schiigerl... 

Targonski, Z. and Pielecki, J., Continuous semi-solid cultivation for the production of cellulase by Trichoderma reesei mutants using a polyurethane foam carrier and a liquid medium, Acta-Biotechnol. 1995 vol. 15, no. 3, pp. 289-296. 

Tuerker. M. and Mavituna, E, Production of cellulase by freely suspended and immobilized cells of Trichoderma reesei, Enzyme-Microb.-Technol. 1987. vol. 9, no. 

Targonski and Pielecki investigated the production of cellulase using immobilized mycelium of Trichoderma reesei mutants on polyurethane foam impregnated... 

Tuerker and Mavituna immobilized Trichoderma reesei within the open porous networks of reticulated polyurethane foam matrices. Growth pattern, glucose consumption, and cellulase production were compared with those of freely suspended cells. The method of immobilization was simple and had no detrimental effect on cell activity. Hundreds of similar projects could be cited. Not all rated the use of polymethane as the preferred technique. If a statistical analysis were conducted on aU the immobilization literature, we are sure that no single technique would be dominant. However, the combination of ease of immobilization, cost of materials, flow-through properties, control of flux rate through the immobilizing membrane, high surface-to-volume ratio, and other factors make polymethane a viable substratum for the continuous production of proteins. 

Figure 7. Production of glucose and cellobiose from Avicel. The reaction mixture contained 1% Avicel PH-101, 1% Trichoderma reesei QM 9123 cellulose protein produced in a pH 3.6 culture medium, and 0.05M sodium acetate buffer, pH 5.0. Incubation conditions and analysis are...

Dynamics of Cellulase Production by Glucose Grown Cultures of Trichoderma reesei Rut-C30 as a Response to Addition of Cellulose... 

Index Entries Cellulase production Trichoderma reesei RUT C30 pH profiling p-glucosidase shake flask fermentor. 

Trichoderma reesei RUT C30 is known to be one of the best hyperpro-ducing cellulolytic fungi. Several factors, such as the amount and quality of carbon source, temperature and pH of the cultivation, and aeration, influence enzyme production of this strain. It has been indicated in previous studies that pH and the pH-controlling strategy have a great effect on the amount of cellulase produced (1-9). 

The filament thickness can be another interesting feature. Production of cephalosporin by Cephalosporium acremonium has been observed in conjunction with some swelling of the hyphae [152]. It has been often reported that, when cultivated on solid substrate, apical parts of filamentous species tend to become thinner, apparently due to substrate limitation, which induces the cell to increase its surface to volume ratio. Similar behavior has been observed in suspended media on fungi (Trichoderma reesei [151] and S. ambofaciens [153, 154]) (Fig. 24). Hyphae are often regarded as solid cylinders, and, in order to... 

Medve, J., Karlsson, J., Lee, D., and Tjerneld, F. 1998. Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei Adsorption, sugar production pattern and synergism of the enzymes. Biotech. Bioeng.,59, 621-634. 

Okada, H., Sekiya, T., Yokoyama, K.,Tohda, H., Kumagai, H., and Morikawa, Y. 1998. Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomy-ces pombe and characterization of its products. Appl. Microbiol. Biotechnol., 49, 301-308. 

Cellulases are found in fungi and bacteria. Of commercial interest are fungal enzymes from Aspergillus or Trichoderma and a few bacterial enzymes. They are either used as a multicomponent, which contain all enzyme types and are found in Trichoderma reesei (Hypocrea jecorina), or as a monocomponent enzyme product, which consists of only one of the three types of enzymes. The multicomponent enzyme preparations can be produced from a selected cellulose overproducing strain of the wild-type organism, whereas the monocomponent cellulases are mainly produced in recombinant production systems. 

An enzyme company has now introduced into the market an enzyme preparation produced by Trichoderma reesei that contains a mixture of cellulase, beta-glucanase and pentosanase. The claim is that this new product is tailor made for the baking industry to extend the softness life of bread. 

Other Uses. Rapeseed or canola meal has been used as a fermentation substrate. It has been included as an additive in compost for commercial button mushroom Agaricus bisporus) production with good success (32). Canola meal has also been tested as a substrate for xylanase production by Trichoderma reesei. Results from this work indicate that the hydrolysis of canola meal by this enzyme system might be useful in converting this material to fermentable sugars that could be further processed to expensive end products such as solvents and chemicals (33). 

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