Trichoderma reesei cellulase system

Production of Trichoderma reesei Cellulase System with High Hydrofytic Potential by Solid-State Fermentation... 

Table V. Composition of Cellulase System Produced on Pro-cell with Trichoderma reesei QMY-1 in SSF...

Cellulases are found in fungi and bacteria. Of commercial interest are fungal enzymes from Aspergillus or Trichoderma and a few bacterial enzymes. They are either used as a multicomponent, which contain all enzyme types and are found in Trichoderma reesei (Hypocrea jecorina), or as a monocomponent enzyme product, which consists of only one of the three types of enzymes. The multicomponent enzyme preparations can be produced from a selected cellulose overproducing strain of the wild-type organism, whereas the monocomponent cellulases are mainly produced in recombinant production systems. 

Claeyssens, M., van Tilbeurgh, H., Tomme, R, Wood, T.M., and McCrae, S. I., Fungal cellulase systems Comparison of the specificities of the cellobiohydrolases isolated from Penicillium pinophilum and Trichoderma reesei. Biochem. J. 1989,261, 819-826. 

The second step in the direct ethanol process is that of enzyme production. The Gulf process utilizes a mutant strain of Trichoderma reesei, grown continuously to produce a complete cellulase system. The residence time is 48 hours. Enzyme production begins on a spore plate with subsequent scale-up to the enzyme production vessel size to be used. Our pilot plant facility has 300-gal enzyme reactors. 

The principal features of a mathematical model described for the enzymatic hydrolysis and fermentation of cellulose by Trichoderma reesei are the assumption of two forms of cellulose (crystalline and amorphous), two sugars (cellobiose and D-glucose), and two enzymes (cellulase and j3-D-glucosidase). An inducer-repressor-messenger RNA mechanism is used to predict enzyme formation, and pH effects are included. The model consists of 12 ordinary differential equations for 12 state variables and contains 38 parameters. The parameters were estimated from four sets of experimental data by optimization. The results appear satisfactory, and the computer programs permit simulation of a variety of system changes. 

Kubicek CP. (2013). Systems biological approaches towards understanding cellulase production by Trichoderma reesei. J Biotechnol, 163, 133-142. 

Ferreira, S. M. P., A. P. Duarte, J. A. Queiroz, and F. C. Domingues. 2009. Influence of Buffer Systems on Trichoderma Reesei Rut C-30 Morphology and Cellulase Production. Electronic Journal of Biotechnology 12 (3). 

Dunne CP (1982) Relationship between extracellular proteases and the cellulase system of Trichoderma reesei. In Chibata J, Fukui J, Wingard Jr LB (eds.) Enzyme Engineering Vol 6. Plenum Press New York p 355... 

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