Transcription in vivo

The Role of PARP-1 as a Modulator of Chromatin Structure and Chromatin-dependent Transcription in vivo Studies... 

Oct-1 and 2 138 Yes. POU homeodomain interacts with Increased transcription in vivo (transfection assay)... 

Association of HMGN proteins with transcription in vivo... 

Prelich, G. and Winston, F. (1993) Mutations that suppress the deletion of an upstream activating sequence in yeast involvement of a protein kinase and histone H3 in repressing transcription in vivo. Genetics 135, 665-676. 

Ko HP, Okino ST, Ma Q, etal. 1996. Dioxin-induced CYP1A1 transcription in vivo The aromatic hydrocarbon receptor mediates transactivation, enhancer-promoter communication, and changes in chromatin structure. Mol Cell Biol 16 430-436. 

Arndt, K.M., Ricupero-Hovasse, S., and Winston, F., 1995, TBP mutants defective in activated transcription in vivo. EMBOJ. 14 1490-1497. 

K.M. Arndt, S.L. Ricupero, D.M. Eisenmann and F. Winston. Biochemical and genetic characterization of a yeast TFIID mutant that alters transcription in vivo and DNA binding in vitro. Mol CeU Biol 12 (1992) 2372-82. 

G.O. Bryant, L.S. Martel, S.K. Burley and A.J. Berk. Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo. Genes Dev. 10... 

Chao SH, Price DH. 2001. Flavopiri-dol inactivates P-TEFb and blocks most RNA polymerase II transcription in vivo. J. Biol. Chem. 276 31795-99... 

Bouvet, P., Dimitrov, S., and Wolffe, A.P. (1994). Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone HI. Genes and Development 5 1147-1159. 

BOX 29.1. TRANSIENT COTRANSFECTION ASSAYS CAN BE USED TO STUDY TRANSCRIPTION IN VIVO... 

Chang TK, Lee WB, Ko HH (2000) trans-Resveratrol modulates the catalytic activity and mRNA expression of the procarcinogen-activating human cytochrome P450 IBl. Can J Physiol Pharmacol 78 874-881 Chang WH, Chen CH, Gau RJ, Lin CC, Tsai CL, Tsai K, Lu FJ (2002) Effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction. Planta Med 68 302-306 Chao SH, Price DH (2001)Flavopitidol inactivates P-TEFb and blocks most RNA polymerase 11 transcription in vivo. J Biol Chem 276 31793-31799... 

Yuli FE, Han W, Jansen ED, Everhart MB, Sadikot RT, Christman JW, Blackwell TS. Bioluminescent detection of endotoxin effects on HIV-1 LTR-driven transcription in vivo. J Histochem Cytochem 2003 51(6) 741-749. 

Enhancer elements that stimulate transcription in vivo at adjacent promoters are thought to be important in regulation of genes during differentiation. The mechanism by which such an element can enhance transcription at promoters positioned over 1000 base pairs away is not known. Some have suggested that enhancer elements may be sites of entry for pol II, it being subsequently transferred to the ini-... 

Alterations in RNA polymerase II transcription are common during replication of most viruses. In some cases, the cellular RNA polymerase is a competitor for the free pool of nucleoside triphosphates and, thus, inhibition of its activity benefits viral replication. In other cases, viral DNA is transcribed by the cellular polymerase II, thus, redistribution of its activity is necessary. Factors necessary for transcription by polymerase II in cells are currently being identified and subsequently purified. The most critical aspect of this work is to identify factors that recognize the sequence elements known to affect transcription in vivo. Alterations in activities of either the factors or the polymerase will probably be a common event in virus-infected cells. In fact, understanding how various viruses modulate transcription by RNA polymerase II will probably be the primary means of establishing the in vivo function of the factors isolated by in vitro assays. Research in this area has only recently begun and its future appears to be rich with new discoveries and insights. 

Fromm, M., and Berg, P., 9S3a, Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen, J. Mol. Appl. Gen. 2 127-135. 

Grosschedl, R., and Birnstiel, M. L., 1980, Spacer DNA sequences upstream of the TATAAATA sequence are essential for promotion of H2A histone gene transcription in vivo, Proc. Natl. Acad. Sci. USA 77 7102-7106. 

---