Carbon-13 tracer

Glaser and Lichtenstein (G3) measured the liquid residence-time distribution for cocurrent downward flow of gas and liquid in columns of -in., 2-in., and 1-ft diameter packed with porous or nonporous -pg-in. or -in. cylindrical packings. The fluid media were an aqueous calcium chloride solution and air in one series of experiments and kerosene and hydrogen in another. Pulses of radioactive tracer (carbon-12, phosphorous-32, or rubi-dium-86) were injected outside the column, and the effluent concentration measured by Geiger counter. Axial dispersion was characterized by variability (defined as the standard deviation of residence time divided by the average residence time), and corrections for end effects were included in the analysis. The experiments indicate no effect of bed diameter upon variability. For a packed bed of porous particles, variability was found to consist of three components (1) Variability due to bulk flow through the bed... 

Expired air. For 14C-labeled chemicals, the tracer carbon may be incorporated in vivo into carbon dioxide, a possible metabolic product. Therefore, when the position of the radiolabel indicates the potential for biological instability, a pilot study to collect expired air and monitor its radioactivity content should be conducted prior to initiating a full-scale study. Expired air studies should also be performed in situations where the radiolabel has been postulated to be stable but analyses of urine and feces from the toxicokinetic study fail to yield complete recovery (mass balance) of the dose. 

Isotopic Tools Tracers. Carbon has three stable or long-lived isotopes 98.9% of earth s C is 12C, -1.1% is 13C (a stable isotope), and about one in a trillion (1 in 1012) carbon atoms is 14C. By enriching or depleting the ratios of the rare isotopes in plants, plant litter, or other organic material put in soil, it is possible to follow the pulse of altered isotopic ratios (and the carbon compounds they were associated with) as they move through the system. 

Krysell M., Fogelqvist E., and Tanhua T. (1994) Apparent removal of the transient tracer carbon-tetrachloride from anoxic seawater. Geophys. Res. Lett. 21, 2511-2514. 

The problem all of these, seemingly contradictory views concerning the mechanism of dehydrogenation of cyclohexane was brightened by use of the method of tracers (carbon-14) in our studies together with Isagulyants and Derbentsev (91). 

In most cases, the carbon due to the tissue is much greater than the carbon due to the tracer, so that the tracer carbon can be neglected in the calculation ... 

Additional evidence for the aldolase reaction being involved in monosaccharide biosynthesis came from the isotope studies of photosynthesis. During short-term photosynthesis, the radioactivity from carbon dioxide appeared in a number of compounds in the following order D-glyceric acid 3-phosphate, triose phosphate, hexose phosphates, and sucrose 70), The distribution of the tracer carbon in these compounds was compatible with... 

When tracer carbon became available, our interest in CO2 and the mechanism by which it is utilized naturally lead us to the use of this tool. We found the tracer in the carboxyls of succinate and from that and other results proposed that CO2 is fixed as follows ... 

The mechanism of the reaction la not known with certainty. It is known from studies utilising as tracer that no change in the carbon skeleton occurs during the reaction, and also that unsaturated hydrocarbons can undergo reactions very similar to those of ketones thus both styiene and phenyl-acetylene can react with sulphur and morpholine to produce phenylaceto-thiomorphoUde, hydrolysis of which yields phenylacetic acid ... 

The changeover from ROO radicals to HOO radicals and the switch from organic peroxides to HOOH has been shown as temperature is increased in propane VPO (87,141). Tracer experiments have been used to explore product sequences in propane VPO (142—145). Propylene oxide comes exclusively from propylene. Ethylene, acetaldehyde, formaldehyde, methanol, carbon monoxide, and carbon dioxide come from both propane and propylene. Ethanol comes exclusively from propane. 

Any radioactive nucUde or isotope of an element can be used as a radioactive tracer, eg, chromium-51 [14392-02-0] cobalt-60 [10198-40-0] tin-110 [15700-33-1] and mercury-203 [13982-78-0],hut the preponderance ofuse has been for carbon-14 [14762-75-5],hydj ogen-3 [10028-17-8] (tritium), sulfur-35 [15117-53-0], phosphoms-32, and iodine-125 [14158-31 -7]. More recendy phosphoms-33 has become available and is used to replace sulfur-35 and phosphoms-32 in many appUcations. By far the greater number of radioactive tracers produced are based on carbon-14 and hydrogen-3 because carbon and hydrogen exist in a large majority of the known natural and synthetic chemical compounds. 

The radioactive isotopes available for use as precursors for radioactive tracer manufacturing include barium [ C]-carbonate [1882-53-7], tritium gas, p2p] phosphoric acid or pP]-phosphoric acid [15364-02-0], p S]-sulfuric acid [13770-01 -9], and sodium [ I]-iodide [24359-64-6]. It is from these chemical forms that the corresponding radioactive tracer chemicals are synthesized. [ C]-Carbon dioxide, [ C]-benzene, and [ C]-methyl iodide require vacuum-line handling in weU-ventilated fume hoods. Tritium gas, pH]-methyl iodide, sodium borotritide, and [ I]-iodine, which are the most difficult forms of these isotopes to contain, must be handled in specialized closed systems. Sodium p S]-sulfate and sodium [ I]-iodide must be handled similarly in closed systems to avoid the Uberation of volatile p S]-sulfur oxides and [ I]-iodine. Adequate shielding must be provided when handling P P]-phosphoric acid to minimize exposure to external radiation. 

Half-inch copper tubing is commonly used for steam tracing. Three-eighths-inch tubing is also used, but the effective circuit length is then decreased from 150 feet to about 60 feet. In some corrosive environments, stainless steel tubing is used, and occasionally standard carbon steel pipe (one half inch to one inch) is used as the tracer. 

Be, and °Be. Radioisotopes of hydrogen and carbon are of particular interest because they are major biological elements, and thus can be used as both chemical and biological tracers. Recently, particle reactive Be = 53.3 day) has been utilized as a valuable coastal tracer of short-term particle deposition and remobilization. ... 

Although many overall rearrangements can be formulated as a series of 1,2-shifts, both isotopic tracer studies and con utational work have demonstrated foe involvement of other species. These are bridged ions in which hydride or alkyl groups are partially bound to two other carbons. Such structures can be transition states for hydride and alkyl-group shifts, but some evidence indicates that these structures can also be intermediates. 

Applications that have been tested with good results are cases where nitrous oxide has been the air contaminant of current interest or used as a tracer gas. Other applications have been carbon disulfide in a rayon factory and styrene vapor, one of the volatile components from a surface-coating material. 

Heavy isotopes endow the compounds in which they appear with slightly greater masses than their unlabeled counterparts. These compounds can be separated and quantitated by mass spectrometry (or density gradient centrifugation, if they are macromolecules). For example, O was used in separate experiments as a tracer of the fate of the oxygen atoms in water and carbon dioxide to determine whether the atmospheric oxygen produced in photosynthesis arose from HgO, COg, or both ... 

Even though acetate units, such as those obtained from fatty acid oxidation, cannot be used for net synthesis of carbohydrate in animals, labeled carbon from " C-labeled acetate can be found in newly synthesized glucose (for example, in liver glycogen) in animal tracer studies. Explain how this can be. Which carbons of glucose would you expect to be the first to be labeled by "Relabeled acetate ... 

The hetero ring in 4-(l -hydroxyalkylidene)-5-oxazolones is cleaved by alcoholic HCl to form alkyl a-acylaminoacylacetates, which cyclize to oxazole-4-carboxylates [Eq. (25)]. This rearrangement occurs directly in alkali, and a carbon-14 tracer study has substantiated a mechanism involving ring opening followed by the alternative ring closure. ... 

The bare tracer is usually copper tubing, or sometimes carbon or stainless steel tubing, usually of -in., V2 -in., or... 

The Use of Carbon 14 as a Tracer to Study the Origin of Tetranitromethane in TNT Manu-... 

In the production of TNT from the reaction between toluene and mixed acids (nitric/sulfuric), TeNMe forms in amounts between 0.2—0.4% of the total wt of TNT. This TeNMe has been held responsible for several expins which have occurred in TNT plants, causing fatal injuries to personnel and severe damage to facilities. These expins were attributed to the presence of TeNMe in the acid fume lines and the acid storage tanks. Mixts of TeNMe and readily oxidizable materials are known to form very powerful and sensitive expl mixts. Since TeNMe is also isolated from the nitration of Nitrobenzene (NB), the TeNMe formed in the nitration of toluene may arise from the oxidation of the aromatic ring and/or methyl group. In an effort to gain more informa-. tion on the origin of TeNMe from TNT production, radioactive carbon-14 (14C) was used as a tracer to determine the extent to which each of the carbon atoms in the toluene skeleton of the various nitro-substituted isomers contributes to... 

Additional 14C-tracer studies were conducted to determine the specific isomer participation for TeNMe formation. In the dinitration mixt there are six isomers present 2,4-, 2,6-, 2,3-, 2,5-, 3,4- and 3,5-Dinitrotoluene (DNT). It was observed that 83% of the TeNMe formed from carbon-1 came from the DNT isomers derived from m-Nitrotoluene. The relative participation of DNT isomers for TeNMe formation is summarized in Table 1... 

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