Total internal reflection fluorescence microscopy TIRFM

To discriminate between surface bound protein molecules and those in bulk solution, total internal reflection fluorescence microscopy (TIRFM)41 55 was employed. TIRFM creates an evanescence wave that decays as a function of distance from the surface as ... [Pg.107]

Complementary microscopy techniques can be used to follow the morphology and growth of fibrils either on a surface or in aliquots taken from the assembly solution including total internal reflection fluorescence microscopy (TIRFM) (Ban et al., 2004), transmission electron microscopy (TEM), or atomic force microscopy (AFM). [Pg.165]

Total Internal Reflection Fluorescence Microscopy (TIRFM) is another useful tool for studying the reactions of individual molecules adsorbed, adhered or bound to surfaces. Typical applications are membrane fusion of vesicles [177], conformational and orientation changes [178] and lateral mobility of molecules [179],... [Pg.142]

Total internal reflection fluorescence microscopy (TIRFM) is a promising alternative approach to low background fluorescence imaging [68], For excitation of molecules on a surface or within a thin slice of the sample, an evanescent optical field is used traveling along the interface between a medium with a high refractive index n, (typically a quartz glass prism) and... [Pg.25]

Total Internal Reflection Fluorescence Microscopy (TIRFM)... [Pg.25]

Total internal reflection fluorescence microscopy (TIRFM), or simply TIRF, is the application of fluorescence imaging to evanescent-wave microscopy. The material of interest is tagged with fluorescent material that emits light at wavelengths greater than the excitation photons from the evanescent field, which additionally improves the detectability of the evanescent-wave microscopy technique. [Pg.3334]

G.A. Truskey, J.S. Burmeister, E. Grapa, W.M. Reichert, Total internal reflection fluorescence microscopy (TIRFM) (II) Topographical mapping of relative celFsubstratum separation distances. J. Cell Sci. 103 (1992), 491-499. [Pg.208]

Abstract Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at tbe single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience. [Pg.77]