TIRFM fluorescence microscopy

To discriminate between surface bound protein molecules and those in bulk solution, total internal reflection fluorescence microscopy (TIRFM)41 55 was employed. TIRFM creates an evanescence wave that decays as a function of distance from the surface as ... 

TIRFM total internal reflection fluorescence microscopy TLM thermal lens microscopy... 

Complementary microscopy techniques can be used to follow the morphology and growth of fibrils either on a surface or in aliquots taken from the assembly solution including total internal reflection fluorescence microscopy (TIRFM) (Ban et al., 2004), transmission electron microscopy (TEM), or atomic force microscopy (AFM). 

Fig. 15.1. Schematic representation of amyloid fibrils revealed by total internal reflection fluorescence microscopy, (a) The penetration depth of the evanescent field formed by the total internal reflection of laser light is 150nm for a laser light at 455 nm, so only amyloid fibrils lying parallel to the slide glass surface were observed. (b) Schematic diagram of a prism-type TIRFM system on an inverted microscope. ISIT image-intensifier-coupled silicone intensified target camera, CCD charge-coupled device camera...

Total Internal Reflection Fluorescence Microscopy (TIRFM) is another useful tool for studying the reactions of individual molecules adsorbed, adhered or bound to surfaces. Typical applications are membrane fusion of vesicles [177], conformational and orientation changes [178] and lateral mobility of molecules [179],... 

Total internal reflection fluorescence microscopy (TIRFM) is a promising alternative approach to low background fluorescence imaging [68], For excitation of molecules on a surface or within a thin slice of the sample, an evanescent optical field is used traveling along the interface between a medium with a high refractive index n, (typically a quartz glass prism) and... 

Total Internal Reflection Fluorescence Microscopy (TIRFM)... 

TIRFM TIRM Total internal reflection fluorescence (microscopy)... 

Kihm KD, Banerjee A, Choi CK, Takagi T (2004) Near-wall hindered Brownian diffusion of nanoparticles examined by three-dimensional ratiometric total internal reflection fluorescence microscopy (3-D R-TIRFM). Exp Fluid 37 811-824... 

Total internal reflection fluorescence microscopy (TIRFM), or simply TIRF, is the application of fluorescence imaging to evanescent-wave microscopy. The material of interest is tagged with fluorescent material that emits light at wavelengths greater than the excitation photons from the evanescent field, which additionally improves the detectability of the evanescent-wave microscopy technique. 

Figure 4. Fluorescence microscopy with axial resolution (A) TIRFM with an angle of illumination close to the critical angle c (B) laser scanning microscopy (C) structured illumination (D) image section calculated from structured illumination microscopy. U373-MG glioblastoma cells incubated with the mitochondrial maker rhodamine 123.

G.A. Truskey, J.S. Burmeister, E. Grapa, W.M. Reichert, Total internal reflection fluorescence microscopy (TIRFM) (II) Topographical mapping of relative celFsubstratum separation distances. J. Cell Sci. 103 (1992), 491-499. 

K. Stock, R. Sailer, W.S.L. Strauss, M. Lyttek, R. Steiner, H. Schneckenburger Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) realization and application of a conqjact illumination device, J. Microsc. 211 (2003), 19-29. 

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