Implantation ex in vivo

A polyvinylpyrrolidone coating was also applied to polyethylene pretreated in a cold O2 plasma (RF, 20 L, SOW, 10 min). The plasma treatment induces the attachment of alcohol and ketone groups, and therefore a hydrophilic character develops. Then, the plasma-modified polyethylene substrate is dipped into an aqueous solution of PVP (Kollidon 90 pharmaceutical grade, molecular weight 90 000 Da). A new, stable, hydrophilic substrate is obtained. 

Polystyrene treated with Ar plasma (with Petri dishes as substrates) was also grafted with cellulose derivative polymers [22], then mouse melanoma cells (B16, C3, FIO) were spread onto the treated surfaces. All three cell lines proliferated significantly more slowly after 48 h [23]. Further, we showed that cells aggregated on this modified substrate could rapidly establish gap junctions for communication between the fibronectin of cell membranes and exogenic surfaces [24]. Cell differentiation occurs and can be used in the biological analysis. For example (Fig. 12.3), pyramidal aggregates of melanoma cells (Swiss 3T3) appear on polystyrene substrates coated with cellulose derivatives after 24 h. These 

---