Cells morphologically intact

The effect of crocin on the TNF-a-induced cell death of PC-12 cells is shown in Fig. (6). In serum-free GIT medium conditions, PC-12 control cell morphology remained intact at 24 h (panel A). However, the cells treated for 24 h with TNF-a (500 units/ml) appeared rounded and showed the characteristics of necrotic and/or apoptotic cells (panel B). In the combination with 10 pM crocin, PC-12 cell morphology retained intact neuronal cell morphology at 24 h (panel C). Crocin alone had no effect on the morphology of PC-12 cells (data not shown). 

The cell walls of the vessels in Persea wood have reacted to the coalifying processes in a way that is different from the response of the fiber-tracheid walls, even though the two wall types may lie in juxtaposition. Microscopic study of thin sections, cut on a microtome, show that the vessel walls are yellow-tan in color, anisotropic, and morphologically intact though often deformed. In contrast, the fiber-tracheid walls have been converted to a yellow-brown to orange-brown, isotropic material that exhibits little of the original... 

Molecular Networks in Morphologically Intact Cells and Tissue-Challenge for Biology and Drug Development... 

The present paper summarizes a new approach of target selection by addressing molecular networks directly in morphologically intact cells and tissue. 

In situ hybridization is a specialized type of solid-support assay in which morphologically intact tissue, cells, or chromosomes affixed to a glass microscope slide provide the... 

Endothelial cells located in the vicinity of atherosclerotic lesions, although morphologically intact in the early stages of atherosclerosis, present a number of abnormalities in that they exhibit decreased basal production of NO as well as an impaired responsiveness to endothelium-dependent vasodilators (Drexler et al., 1989). For example, serotonin, which dilates normal human coronary arteries, causes a paradoxical vasoconstriction in... 

M. N. Berry, High-yield preparation of morphologically intact isolated parenchzmal cells from rat liver, in. Methods in Enzymology, Vol. XXXII (S. Fleischer and L. Parker, eds.), pp. 625-632, Academic Press, New York (1974). 

When embryos treated as described above were left to develop, they reached the onset of gastrulation with no visible changes in morphology compared to untreated embryos. However, as they reached stage 10.5, the embryos underwent rapid and synchronous apoptosis. This rapid cell death had striking morphological characteristics individual cells from the embryo lost their cohesion and adhesiveness and rapidly lysed so that few or no intact cells were found in each embryo 20 minutes following the onset of apoptosis (Fig. IB). 

The central unit of these peptidomimetics imitates a /1-turn and brings the NH2-terminus of the cysteine analogue and the CO OH terminus of the methionine in spatial proximity these can then complex the Zn2+ ion which is essential for activity of the FTase [26]. The free acid 7 inhibits the enzyme with an IC50 value of 1 nmol/1, whilst in intact cells the methyl ester 8, despite its weaker in vitro activity, is significantly more potent because it can penetrate the plasma membrane better due to its lower polarity. This property can be used to convert the morphology of H-Ras-transformed cells back to the normal form and to inhibit growth of these cells, whereas the substance shows no effect on Src-transformed and untransformed rat fibroblasts. The inhibitor therefore acts selectively on transformed cells and does not influence growth of normal cells. This result is noteworthy because farnesylation of the wild type H-Ras protein... 

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