Tissue homogenizers

Liquid chromatographic determination of chloramphenicol in kidney tissue homogenates using valve-switching techniques , 113 171 -174(1988). 

Soluble tetrameric form (G4) Composed of four identical monomers and stabilized by hydrophobic interactions of hydrophobic amino acids at the C terminus of monomers. Abundant for brain AChE and BChE in mammalian body fluids and in the soluble fraction of tissue homogenates. 

Blood, tissues Homogenize, if tissue mix sample with acetone centrifuge concentrate saturate with sodium chloride evaporate organic layer cleanup on silica gel eluting with hexane-benzene concentrate GC/FPD <100 ppb No data EPA1980d... 

Tissue Homogenized with saline and isooctane at 4°C headspace gas analyzed GC/ECD 8.4 ppb 86-91 Chenetal. 1993... 

Experiments with aldicarb sulfone in ground beef involved simple extraction with acetonitrile during tissue homogenization and resulted in a definite immunoassay response at the tolerance level of 10 qg kg A moderate, but rather consistent, matrix effect was observed. A more severe matrix effect was observed in bovine milk, blood, and urine. For the liquid matrices, sample dilution was not a satisfactory strategy, because the assay variability increased at lower concentrations, negating any benefit of reducing the matrix effect. This work clearly demonsttated that matrix effects are... 

Ivermectin, a macrocyclic lactone, is also utilized to control parasites. An immunoassay was developed to determine ivermectin residues in bovine liver by Crooks etal. The sample preparation procedure was complex, involving tissue homogenization in acetonitrile, centrifugation, extraction with hexane (to remove lipids), evaporation and reconstitution in ethyl acetate, and passage through an SPE column followed... 

Western blot A method to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. Some diagnostic applications for the Western blot include Lyme disease, bovine spongiform encephalopathy, and human immunodeficiency virus (HIV) (it is considered the gold standard for HIV diagnostic testing). 

Human adipose tissue Homogenization solvent extraction GPC and Florisil column clean-up Capillary GC/NPD confirmation by GC/MS Low ng/g 69-104% LeBel and Williams 1983... 

Brain tissue Homogenization in coid 0.01 M NaOH. Centrifuge and resuspend pellet add zinc acetate and ascorbic acid readjust pH use continuous flow gas dialysis system to separate sulfide. Gas dialysis/ion chromatography with ECD 0.02 pg/g 95-100% Goodwin et al. 1989... 

Moreover, experimental reference maps of human tissues were studied p7 and Mr coordinates of identified spots were retrieved from the SWISS-2D-PAGE database, the values <3X = 0.009 pH and av = 0.0002 log Mr were assumed for spot dimension since they represent the standard case for experimental 2D-PAGE maps—normal sample loading of a tissue homogenate (ca. 1 mg total protein) and standard gel sizes (18 x 20 cm, IEF x SDS-PAGE). 

Clinical specimens obtained for the recovery of dematiaceous fungi usually do not require extensive processing. If aspirated specimens contain a substantial amount of purulent material, this can be dissolved with N-acetyl-L-cysteine without sodium hydroxide. Tissue specimens and biopsy material should be homogenized in a tissue homogenizer after highly suspicious areas consisting of necrotic, purulent, or caseous material are selectively examined microscopically and inoculated onto isolation media. 

Edgar PF Comparative proteome analysis. Tissue homogenate from normal human hippocampus subjected to two-dimensional gel electrophoresis and Coo-massie blue protein staining. Mol Psychiatry 2000 5 85-90. 

The cyclic voltammetry procedure reported by Kohen and others (2000) evaluates the overall reducing power of low-molecular-weight antioxidants in a biological fluid or tissue homogenate. The electrochemical oxidation of a certain compound on an inert carbon glassy electrode is accompanied by the appearance of the current at a certain potential. While the potential at which a cyclic voltammetry peak appears is determined... 

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