Tissue homogenates oxidative phosphorylation

Working with hypertonic sucrose homogenates, Kennedy and Lehninger found that isolated washed mitochondria of rat liver tissue oxidized not only succinate but also other key intermediates of the Krebs cycle. They also reported that fatty acid oxidation was localized in the mitochondria and furthermore demonstrated that phosphorylations coupled to these oxidations took place in these preparations, as could be shown with tracer phosphate experiments. 

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