Luminescence time-resolved imaging

Nagl S (2004) Quantitative determination of proteins on microarrays by time-resolved luminescent imaging. Diploma thesis, University of Regensburg... 

In addition. Yuan and coworkers described spherical silica-based luminescent europium nanoparticles with a uniform size of about 10 nm in diameter [87]. Interestingly, the nanoparticles can be excited over a wavelength range from the UV to visible light (200 50 nm) in aqueous solution, and could be used for time-resolved luminescence imaging of an environmental pathogen, Giardia lamblia. 

Weibel N, Charbonniere LJ, Guardigli M, Roda A, Ziessel R. Engineering of highly luminescent lanthanide tags suitable for protein labeling and time resolved luminescence imaging. J Am Chem Soc 2(X)4 126 4888-96. 

Figure 17 Time-resolved luminescence images of Uving HeLa cells loaded with different Ln " complexes, (a). Cells were incubated in the presence of 100 rM [Eu2(L )3] and [Tb2(L )3] in RPMl-1640 for 6 hat 37°C (A) bright field (B) Tb luminescence (excitation 340nm (Band pass, BP 70mn) filter emission 545 (BP 35nm) filter delay time 100 xs 60 s exposure time) (C) Eu luminescence (excitation 340 nm (BP 70 nm) filter emission 585 nm LP filter delay time 100 xs 60 s exposure time) (D) merged images, (b). HeLa cells were incubated in the presence of 500 xM [Sm2(L )3] in RPMl-1640 for 24 h at 37 °C Sm luminescence (excitation 340 nm (BP 70nm) filter emission 420nm LP filter, delay time 50 xs 60 s exposure time). (Taken from Ref 40. Reproduced by permission of The Royal Society of Chemistry.)...

Murdock R. H., E. R. Menzel. A computer interfaced time-resolved luminescence imaging system, J. Forens. Set, 38, 521-529 (1993). 

An example of the utility of the time-resolved technique in eliminating the interference from background fluorescence in bioimaging is shown in Figure 13.17b. Nagano and coworkers compared time-resolved luminescence microscopy with conventional microscopy using live cultured HeLa cells injected with a Eu + complex Eu-36 (or Eu-37). In the prompt fluorescence images, both the luminescence of Eu-36 (or Eu-37) and weak autofluorescence from... 

Deiters, E., Song, B., Chauvin, A.S., et al. (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-Ught range. Chemistry —A European Journal, 15, 885—900. Charbonniere, L.J., Weibel, N., Estoumes, C., et al. (2004) Spatial and temporal discrimination of siUca particles functionalized with luminescent lanthanide markers using time-resolved luminescence microscopy. New Journal of Chemistry, 28, 777—781. 

Rajapakse H. E., N. Gahlaut, S. Mohandessi, D. Yu, J. R. Turner, L. W. Miller. Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells, Proc. Natl Acad. Sci. USA, 107, 13582-13587 (2010). 

Zhang L., L. Tian, Z. Ye, B. Song, J. L. Yuan. Preparation of visible-light-excited europium biolabels for time-resolved luminescence cell imaging application, Talanta, 108,143-149 (2013). 

Marriott, G., Heidecker, M., Diamandis, E. P. and Yan-Marriott, Y. (1994). Time-resolved delayed luminescence image microscopy using an europium ion chelate complex. Biophys. J. 67, 957-65. 

Steinkamp [91] for the Luminex microspheres. Recently, Mayr et al. stained magnetic microspheres with luminescent ruthenium(II) metal ligand complexes [92] which were encoded by time resolved imaging in the microsecond domain. 

Table 1 Luminescence properties of oxygen-sensitive probes applicable to time-resolved imaging...

The combination of different fluorescent metal indicators with inert luminescent reference beads consisting of poly(acrylonitrile) containing Ru(dpp)3 leads to a sensor array in a microwell plate format, suited for ratiomet-ric time-resolved imaging [95]. The data can be acquired with the help of the f-DLR method (for details see Sect. 2.3). A cross-reactive sensor array was arranged for the determination of mixtures of calcium(II), copper(II), nickel(II), cadmium(II), and zinc(II) ions by nine different commercially available fluorescent indicators (Table 3). For a successful application, it is mandatory that all luminophores can be excited at the same wavelength range between 400 and 500 nm, and that the excitation and emission spectra of all indicators overlap with those of the reference dye encapsulated in the nanobeads. 

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