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Time-resolved luminescence microscopy

Figure 17 Experimental setup for time-resolved luminescence microscopy with lanthanide ions. Figure 17 Experimental setup for time-resolved luminescence microscopy with lanthanide ions.
Charbonniere, L. Ziessel, R. Guardigli, M. Roda, A. Sabbatini, N. Cesario, M. Lanthanide tags for time-resolved luminescence microscopy displaying improved stability and optical properties. J. Am. Chem. Soc. 2001,123, 2436-2437. [Pg.422]

An example of the utility of the time-resolved technique in eliminating the interference from background fluorescence in bioimaging is shown in Figure 13.17b. Nagano and coworkers compared time-resolved luminescence microscopy with conventional microscopy using live cultured HeLa cells injected with a Eu + complex Eu-36 (or Eu-37). In the prompt fluorescence images, both the luminescence of Eu-36 (or Eu-37) and weak autofluorescence from... [Pg.542]

Deiters, E., Song, B., Chauvin, A.S., et al. (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-Ught range. Chemistry —A European Journal, 15, 885—900. Charbonniere, L.J., Weibel, N., Estoumes, C., et al. (2004) Spatial and temporal discrimination of siUca particles functionalized with luminescent lanthanide markers using time-resolved luminescence microscopy. New Journal of Chemistry, 28, 777—781. [Pg.569]

Song B, Vandervyver CDB, Chauvin AS, Bunzli JCG (2(X)8) Time-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells. Org Biomol Chem 8 4125-4133... [Pg.180]

TRLM time-resolved luminescence microscopy or microscope... [Pg.184]

Song B., C. D. B. Vandevyver, A.-S. Chauvin, J.-C. G. BtinzU. Time-resolved Luminescence Microscopy of Bimetalhc Lanthanide Helicates in Living Cells, Org. BiomoL Chem., 6,4125-4133 (2008). [Pg.194]

Charbonniere LJ, Hildebrandt N, Ziessel RF, Lohmannsroben HG (2006) Lanthanides to quantum dots resonance energy transfer in time-resolved fluoro-immunoassays and luminescence microscopy. J Am Chem Soc 128 12800-12809... [Pg.24]

Marriott, G., Heidecker, M., Diamandis, E. P. and Yan-Marriott, Y. (1994). Time-resolved delayed luminescence image microscopy using an europium ion chelate complex. Biophys. J. 67, 957-65. [Pg.71]

Hanaoka, K., Kikuchi, K., Kobayashi, S., and Nagano, T. (2007) Time-resolved long-lived luminescence imaging method employing luminescent lanthanide probes with a new microscopy system. Journal of the American Chemical Society, 129, 13502-13509. [Pg.567]

Luminescent RE + chelates have been successfully developed as labels and probes for highly sensitive and selective bioassays in the past two decades. Time-resolved Inmines-cence detection has been widely applied in fluoroimmunoassay, DNA hybridization assay, enzyme assay, cell activity assay and fluorescence imaging microscopy. ... [Pg.172]

The main luminescence parameters traditionally measured are the frequency of maximal intensity Vmax, intensity I, the quantum yield < >, the hfetime of the exited state T, polarization, parameters of Raman spectroscopy, and excited-state energy migration. The usefulness of the fluorescence methods has been greatly enhanced with the development of new experimental techniques such as nano-, pico-, and femtosecond time-resolved spectroscopy, single-molecule detection, confocal microscopy, and two-photon correlation spectroscopy. [Pg.67]

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