Thermophilicity

Thermooxidatiion Thermopave Thermophilic bacteria Thermophotometry Thermopiles Thermoplastic... 

The demonstration unit was later transported to the CECOS faciHty at Niagara Falls, New York. In tests performed in 1985, approximately 3400 L of a mixed waste containing 2-chlorophenol [95-57-8] nitrobenzene [98-95-3] and 1,1,2-trichloroethane [79-00-5] were processed over 145 operating hours 2-propanol was used as a supplemental fuel the temperature was maintained at 615 to 635°C. Another 95-h test was conducted on a PCB containing transformer waste. Very high destmction efficiencies were achieved for all compounds studied (17). A later bench-scale study, conducted at Smith Kline and French Laboratories in conjunction with Modar (18), showed that simulated chemical and biological wastes, a fermentation broth, and extreme thermophilic bacteria were all completely destroyed within detection limits. 

Thermoduric, Thermophilic, andPsychrophihc Bacteria. Thermoduric bacteria survive but do not grow at pasteurization temperatures. They are largely non spore-forming, heat-resistant types that develop on surfaces of unclean equipment. These bacteria are determined by subjecting a sample to laboratory pasteurization and examining it by the agar plate method. 

Thermophilic bacteria are able to grow at 55°C. They are spore-forming bacilli that can enter milk from a variety of farm sources. Tbermophiles grow ia milk held at elevated temperatures. Their presence ia milk is determined by means of the agar plate method and iacubation at 55°C. 

Yogurt. Yogurt is a fermented milk product that is rapidly increasing in consumption in the United States. Milk is fermented with Uactobacillus bulgaricus and Streptococcus thermophilous organisms that produce lactic acid. Usually some cream or nonfat dried milk is added to the milk in order to obtain a heavy-bodied product. 

A strain of thermophilic Acetobacter - 9. patented in Japan for oxidizing ethanol in a submerged culture oxidizer at temperatures as high as 37°C with considerable savings in cooling water. Another theimophilic strain oiyAcetobacter xn.2An.. 2cm.ed full activity at 35°C, and 45% of its maximum activity at 38°C. 

Chirazymes. These are commercially available enzymes e.g. lipases, esterases, that can be used for the preparation of a variety of optically active carboxylic acids, alcohols and amines. They can cause regio and stereospecific hydrolysis and do not require cofactors. Some can be used also for esterification or transesterification in neat organic solvents. The proteases, amidases and oxidases are obtained from bacteria or fungi, whereas esterases are from pig liver and thermophilic bacteria. For preparative work the enzymes are covalently bound to a carrier and do not therefore contaminate the reaction products. Chirazymes are available form Roche Molecular Biochemicals and are used without further purification. 

Summation of mesophilic bacteria, fungi, and thermophilic bacteria. CPU = colony-forming units. 

Composting is the process of aerobic thermophilic decomposition of organic wastes to a relatively stable humus. Decomposition results from the biological activity of microorganisms which exist in the waste. 

As already stated, aggression will also depend on soil Eh according to the scale given in Table 2.21. Temperature is also a controlling factor and both psychrophylic (cold) and thermophilic (hot) forms are known, e.g. in electrical transformers, hot water systems. 

The subsequent advance was rather fortuitous and rested more with serendipity than with scientific logic. A search was made for cheaper more effective replacements for casein hydrolysate. Amongst the tested materials was com steep liquor (CSL). CSL is a by-product of the manufacture of starch from maize kemals. Whole maize is incubated in warm water, at 50°C acidified with SO2. Thermophilic bacteria hydrolyse proteins and other components of the kemals, thereby loosening the starch granules. These are removed, leaving behind the steep liquor which is used to treat further maize kemals. Ultimately, the liquor is too viscous to re-use and the liquor is concentrated and used as cattle feed. It was this material that was used for penicillin fermentation. Surprisingly, the yield of penicillin increased by a further 5-10 fold giving yields of 50-100 ig ml. 

Brock, T.D. Thermophilic Microorganisms and Life at Hleh Temperatures Springer-Verlag New York, 1978 pp 149-152. 

This key enzyme of the dissimilatory sulfate reduction was isolated from all Desulfovibrio strains studied until now 135), and from some sulfur oxidizing bacteria and thermophilic Archaea 136, 137). The enzymes isolated from sulfate-reducing bacteria contain two [4Fe-4S] clusters and a flavin group (FAD) as demonstrated by visible, EPR, and Mossbauer spectroscopies. With a total molecular mass ranging from 150 to 220 kDa, APS reductases have a subunit composition of the type 012)32 or 02)3. The subunit molecular mass is approximately 70 and 20 kDa for the a and )3 subunits, respectively. Amino-acid sequence data suggest that both iron-sulfur clusters are located in the (3 subunit... 

Metalloenzymes and Redox Proteins from Extremely Thermophilic Bacteria... 

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