Tetrazolium chloride, 2,3,4-triphenyl

TPTZ 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate TTC... 

Triphenylstannium acetate. See Fentin acetate Triphenylstibine. See Triphenylantimony 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate. See Tetrazolium chloride Triphenyl thiophosphate 0,0,0-Triphenyl thiophosphate. See Triphenyl phosphorothionate... 

Phytochemical reduction of tetrazolium salts has been observed by Kuhn and Jerschel. If 2,3,5-triphenyl tetrazolium chloride is added to fermenting yeast the corresponding formazan is formed. Like most formazyl compounds it has a red color. 

Few examples of photochemical dehydrogenation reactions accompanied by cyclization are reported in the literature. 2,3,5-Triphenyl-tetrazolium chloride (CV), which is used biochemically as reduction indicator, on irradiation in ethanol with ultraviolet light, undergoes dehydrogenation accompanied by cyclization to yield 2,3 (2,2 -bi-phenylene)-5-phenyltetrazolium chloride (CVI).106 167 Light-induced cyclization of substituted derivatives of CV to the corresponding bi-phenylene derivatives has been reported recently. sl 33... 

The bacterial toxicity was measured according DEV, DIN 38412, L3 (TTC(2,3,5-triphenyl-2/7-tetrazolium chlorid) test). The result gives an EC50 (effective concentration) varying between 2290 and 14700 mg/L, and estimated NOEC values in the range of < 10 to 100 mg/L [3.223],... 

Kuhn et al. found that triphenylverdazyl (61) was produced by reaction of 2,3,5-triphenyl-tetrazolium chloride (284) with diazomethane (66M846). 

Several calorimetric methods were reported for spironolactone using different derivatization reagents, such as thiosemicarbazide at 440 nm [24], 4-dinitrobenzene at 400 nm [25], isoniazide at 375 nm [26], acetic acid-acetic anhydride then H2SO4 at 500 nm [27], 2,3,5-triphenyl-tetrazolium chloride and tetramethylammonium hydroxide at 480 nm [28] sodium nitroprusside at 700 nm, and phosphotungestic acid at 410 nm [29]. 

In contrast with previous LTV spectrophotometric method, Khan et al. [17] reported first -derivative spectrophotometric method to determine glimepiride in pharmaceutical dosage form. In this method, formation of drug complex was carried out by reacting glimepiride in MeOH with 2,3,5-triphenyl-2H-tetrazolium chloride in NaOH 0.1 M as an alkaline media. This mixture was then heated at 60 2 °C for 60 min. Drug complex formed was showing maximum absorption at... 

Triphenyl-2H-tetrazolium chloride. Available from Aldrich and Eastman. Light-sensitive. Freshly prepared aqueous solutions should be used in tests. Any unused solution should be acidified and discarded. 

Mercury(II) forms a 1 1 complex with triphenyl-tetrazolium chloride (TTC) that exhibits an absorption maximum at 255 nm. The mercury (II) in a soil sample was extracted into an organic solvent containing an excess of TTC, and the resulting solution was diluted to 100.0 mL in a volumetric flask. Five-milliliter aliquots of the analyte solution were then transfered to six 25-mL volumetric flasks. A standard solution was then prepared that was 5.00 X 10 M in Hg(II). Volumes of the standard solution shown in the table were then pipetted into the volumetric flasks, and each solution was then diluted to 25.00 mL. The absorbance of each solution was measured at 255 nm in 1.00-cm quartz cells. 

Tennant (T7) has also described an automated method using a Technicon continuous flow system and a short incubation time of 4 hours. Growth response of the organism to folate was estimated by measuring the rate of reduction of 2,3,5-triphenyl tetrazolium chloride. 

The heart is excised and cut into 5-mm slices, and stained with 2% triphenyl tetrazolium chloride to histochemically identify the necrotic areas (65). Each slice is further divided into smaller segments for biodistribution studies following a standard protocol (21,43). 

H-Tetrazole-5-thiol, 1-phenyl. See 1-Phenyl-5-mercaptotetrazole Tetrazolium chloride CAS 298-96-4 EINECS/ELINCS 206-071-6 Synonyms PTB Red tetrazolium Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride... 

Tetrazolium red Tetrazolium salt 2H-Tetrazolium, 2,3,5-triphenyl-, chloride. See Tetrazolium chloride... 

To enhance the applicability of UV spectrophotometry in pharmaceutical steroid analysis by increasing its selectivity and sensitivity, various methods have been developed based on chemical reactions leading to colored derivatives. Although their importance has decreased considerably, some of these methods are still in use in pharmacopoeias, mainly in the assay of formulations. For example, the A" -3-oxo and A " -3-oxo steroids can be determined as iso-nicotinoyl hydrazones (/Uax 380 and 410 nm 11500 and 17000, respectively). The dihydroxy-acetone side chain of corticosteroids at C17 reduces tetrazolium reagents to colored formazans (Amax 485 and 525 nm with triphenyl tetrazolium chloride and Tetrazolium Blue reagents, respectively e 16000 and 24000), thus creating the basis for a stability-indicating indirect colorimetric assay. Corticosteroids... 

Different tetrazolium salts have been studied for dosimetry purposes both in solutions and in solid matrices. The 2,3,5-triphenyl-tetrazolium chloride (TTC) solutions and gels are found... 

Table 4. Cytotoxicity of quartz particles with contaminated surfaces established by the reduction of triphenyl tetrazolium chloride (TTC) (from Robock and Klosterkotter 1975)...

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