TTC, 2,3,5-triphenyltetrazolium chloride

TOF-SIMS Time-of-flight secondary ion mass spectrometry TTC 2,3,5-Triphenyltetrazolium chloride... 

Growth conditions in deep-well microtiter plates were optimized with respect to optimal expression of active enzymes (Fig. 2.2.1.1). The best results were obtained with an expression time of 20 h at 37 °C (Fig. 2.2.1.1, lanes 7-9). Subsequently, E. coli cells were enzymatically disrupted by lysozyme treatment, and the carboligase activity was monitored by a modified tetrazolium salt color assay [16], This color assay is based on the reduction of the 2,3,5-triphenyltetrazolium chloride (TTC) 13 to the corresponding formazan 15, which has an intense red color (Fig. 2.2.1.2A). Before screening ofa BFD variant library, substrates and products were tested in the color assay. Neither substrate, benzaldehyde 4 nor dimethoxy-acetaldehyde 8, reduced TTC 13 however, the product 2-hydroxy-3,3-dimethoxy-propiophenone 10 already caused color formation at low concentrations of 2.5-10 mM (Fig. 2.2.1.2B). Benzoin 12 as the product also gave a color change at a similar concentration (data not shown). 

Cerebral infarct volume was evaluated 22 h after reperfusion in rats subjected to 2 h MCAo. Animals were sacrificed by decapitation and the brains were rapidly removed. Eight serial sections from each brain were cut at 2-mm intervals from the frontal pole using a rat brain matrix (Harvard Apparatus, MA, USA). To measure ischemic damage, brain slices were stained in a solution containing 2% 2,3,5-triphenyltetrazolium chloride (TTC) in saline, at 37 °C. After 10 min incubation, the slices were transferred to 10% neutral buffered formaldehyde and... 

TPTZ 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate TTC... 

The cell viability was determined by using 2,3,5-triphenyltetrazolium chloride (TTC) to measure dehydrogenase activity. No difference in cell viability was found between irradiated and unirradiated gingers (Wu and Yang, 1994b). 

Other Names 2,3,5-Triphenyl-2//-tetrazolium chloride 2//-Tetrazolium, 2,3,5-triphenyl-, chloride 1,3,5-Triphenyl-2//-tetrazolium chloride 1,3,5-Triphenyltetra-zolium chloride 2,3,5-Triphenyltetrazolium chloride PTB RT Red tetrazolium TPTZ TT TTC TTC (dye) ... 

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