TRIPHENYL TETRAZOLIUM CHLORIDE TTC

Other Names 2,3,5-Triphenyl-2//-tetrazolium chloride 2//-Tetrazolium, 2,3,5-triphenyl-, chloride 1,3,5-Triphenyl-2//-tetrazolium chloride 1,3,5-Triphenyltetra-zolium chloride 2,3,5-Triphenyltetrazolium chloride PTB RT Red tetrazolium TPTZ TT TTC TTC (dye)  

Tetrazolium chloride Tetrazolium red Triphenyltetrazo-lium chloride Urocheck Uroscreen Vitastain Merck Index Number 9744 Chemical/Dye Class Tetrazolium salt Molecular Formula C19H15CIN4 Molecular Weight 334.80 

Physical Form Colorless needles, turns yellow on exposure to light 

Solubility Soluble in water, ethanol, acetone insoluble in ether 

Staining Applications Bacteria brain sections brain slices cells cerebral infarcts cortex infarct-ed hearts tissues neurons plant roots  

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Mercury(II) forms a 1 1 complex with triphenyl-tetrazolium chloride (TTC) that exhibits an absorption maximum at 255 nm. The mercury (II) in a soil sample was extracted into an organic solvent containing an excess of TTC, and the resulting solution was diluted to 100.0 mL in a volumetric flask. Five-milliliter aliquots of the analyte solution were then transfered to six 25-mL volumetric flasks. A standard solution was then prepared that was 5.00 X 10 M in Hg(II). Volumes of the standard solution shown in the table were then pipetted into the volumetric flasks, and each solution was then diluted to 25.00 mL. The absorbance of each solution was measured at 255 nm in 1.00-cm quartz cells. 

Different tetrazolium salts have been studied for dosimetry purposes both in solutions and in solid matrices. The 2,3,5-triphenyl-tetrazolium chloride (TTC) solutions and gels are found... 

Table 4. Cytotoxicity of quartz particles with contaminated surfaces established by the reduction of triphenyl tetrazolium chloride (TTC) (from Robock and Klosterkotter 1975)...

The bacterial toxicity was measured according DEV, DIN 38412, L3 (TTC(2,3,5-triphenyl-2/7-tetrazolium chlorid) test). The result gives an EC50 (effective concentration) varying between 2290 and 14700 mg/L, and estimated NOEC values in the range of < 10 to 100 mg/L [3.223],... 

TPTZ 2,3,5-Triphenyltetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride 2,3,5-Triphenyl-2H-tetrazolium chloride monohydrate TTC... 

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